Overview

  • Product name
  • Description
    Rabbit polyclonal to ERp29
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non human primates
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Rat ERp29.

  • Positive control
    • WB: MDCK, HepG2, NIH/3T3, A549, MCF7, A431 and HEK-293 membrane-enriched extracts; HEK-293 cell lysate. ICC/IF: HepG2, HMVEC, NS-1 and p19 cells; rat thyrocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab11420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500. PubMed: 17451556

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 17652758
ICC Use at an assay dependent concentration.
WB 1/2500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate.

IP 1/500.
ICC/IF 1/500.

Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.

Target

  • Relevance
    Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
  • Cellular localization
    Endoplasmic reticulum, Cell surface.
  • Database links
  • Alternative names
    • C12orf8 antibody
    • Chromosome 12 open reading frame 8 antibody
    • Endoplasmic reticulum lumenal protein ERp28 antibody
    • Endoplasmic reticulum protein 29 antibody
    • Endoplasmic reticulum protein 29 isoform 1 antibody
    • Endoplasmic reticulum protein ERp29 antibody
    • Endoplasmic reticulum resident protein 28 antibody
    • Endoplasmic reticulum resident protein 29 antibody
    • Endoplasmic reticulum resident protein 31 antibody
    • Epididymis secretory protein Li 107 antibody
    • ERp28 antibody
    • ERP29_HUMAN antibody
    • ERp31 antibody
    • HEL S 107 antibody
    • PDI-DB antibody
    • PDIA9 antibody
    • Protein disulfide isomerase family A member 9 antibody
    see all

Images

  • All lanes : Anti-ERp29 antibody (ab11420) at 1 µg/ml

    Lane 1 : MDCK (canine kidney cell line) membrane-enriched extract
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) membrane-enriched extract
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) membrane-enriched extract
    Lane 4 : A549 (human lung carcinoma cell line) membrane-enriched extract
    Lane 5 : MCF7 (human breast adenocarcinoma cell line) membrane-enriched extract
    Lane 6 : A431 (human epidermoid carcinoma cell line) membrane-enriched extract
    Lane 7 : HEK-293 (human epithelial cell line from embryonic kidney) membrane-enriched extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa



    12% SDS-PAGE

  • Western blot of ERp29 in HEK cell lysate using ab11420.
  • ICC/IF image of ab11420 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11420, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labeling ERp29 with ab11420.

  • Immunocytochemistry/Immunofluorescence analysis of NS-1 cells labeling ERp29 with ab11420.

  • Immunocytochemistry/Immunofluorescence analysis of p19 cells labeling ERp29 with ab11420.

  • Immunocytochemistry/Immunofluorescence analysis of rat thyrocytes labeling ERp29 with ab11420.

References

This product has been referenced in:
  • Song J  et al. Native low density lipoprotein promotes lipid raft formation in macrophages. Mol Med Rep 13:2087-93 (2016). Mouse . Read more (PubMed: 26781977) »
  • Kaneko Y  et al. Kainic Acid-Induced Golgi Complex Fragmentation/Dispersal Shifts the Proteolysis of Reelin in Primary Rat Neuronal Cells: An In Vitro Model of Early Stage Epilepsy. Mol Neurobiol 53:1874-1883 (2016). ICC . Read more (PubMed: 25790952) »
See all 12 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Retinas)
Gel Running Conditions
Non-reduced Denaturing (TGX gel)
Loading amount
10 µg
Specification
Retinas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Prof. Minzhong Yu

Verified customer

Submitted May 09 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - Triton X-100
Specification
Retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Nov 29 2012

Answer

Thank you for your telephone call yesterday afternoon.

I am pleased to provide some information regarding suitable secondaries for the primary antibodies you have selected:

ab48394 LC3B antibody, ab24170 LAMP1antibody andab11420 ERp29 antibody are all rabbit polyclonal. Polyclonal antibodies contain mainly IgG isotypes, soyou need toselect an anti Rabbit IgG tested in IHC-Fr. I have selected some FITC conjugated anti rabbit IgG antibodies that are tested in IHC-Fr. I can suggest to review the online datasheets for further information. Please check that your confocal microscope will detect FITC. Also, if you require any different conjugates, please let me know and I can provide a further list for you as we have many available.

ab6717 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)
Tested in: Flow Cyt, IHC-P, ELISA, IM, ICC/IF, IHC-Fr
https://www.abcam.com/index.html?datasheet=6717 (or use the following: https://www.abcam.com/index.html?datasheet=6717).

ab7086 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed
Tested in: Flow Cyt, IHC-P, ELISA, IM, ICC/IF, IHC-Fr
https://www.abcam.com/index.html?datasheet=7086 (or use the following: https://www.abcam.com/index.html?datasheet=7086).


The ab14705 MTCO1 antibody [1D6E1A8] and ab2861 SERCA2 ATPase antibody [2A7-A1] are both mouse monoclonal IgG2a.

We have no anti mouse IgG2a secondaries tested in IHC-Fr, but in this case you canuse a general anti-mouse IgG.I have selected an FITC conjugated secondary, but again please let me know if you would prefer other conjugates as we have many available.

ab6785 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
Tested in: IHC (PFA fixed), Flow Cyt, IHC-P, WB, ELISA, IM, ICC/IF, IHC-Fr
https://www.abcam.com/index.html?datasheet=6785 (or use the following: https://www.abcam.com/index.html?datasheet=6785).

Both ab2861 and ab14705 are mouse antibodies. These are tested successfully in mouse, and so I would like to reassure you we can provide our guarantee for the results. However, because you are staining mouse tissue, I can suggest it may be better to select primary antibodies that are not raised in mouse. When you add the anti-mouse secondary during the staining, it is possible this can bind to mouse antibodies within the tissue and give some background staining.

You may like to consider the following instead:
ab3625 SERCA2 ATPase antibody
Rabbit polyclonal
Tested in: IHC-P, IP, WB, ICC/IF, IHC-Fr
Reacts with: Hu, Ms, Rat, Pig, Rb

I am sorry there are no alternatives for the MTCO1.

If you require some further information regarding antibodies and IHC, as discussed on the telephone I am including some links from our website. These can be found from the scientific support tab on the homepage:

Antibody guide:
https://www.abcam.com/index.html?pageconfig=resource&rid=11287

Protocols (Select Immunostaining from the list on the following protocolspage):
https://www.abcam.com/index.html?pageconfig=popular_protocols

Fluorochrome guides:
https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf
https://www.abcam.com/index.html?pageconfig=resource&rid=11755

How to choose the right secondary:
https://www.abcam.com/index.html?pageconfig=resource&rid=12825

How to choose a conjugate:
https://www.abcam.com/index.html?pageconfig=resource&rid=12922

And the following external website may also be helpful:
http://www.ihcworld.com/

I hope this will be helpful. If you require any further information or assistance, please do not hesitate to contact us (mailto:technical@abcam.com).

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 whole cell lysate)
Loading amount
25 µg
Specification
PC12 whole cell lysate
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 08 2008

Question
Answer

Thank you for your enquiry. Unfortunately the IgG concentration for ab11420 has not been determined. However, I would estimate that the concentration is between 1-2 mg/ml. I apologize that I could not be of more assistance.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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