Overview

  • Product name

  • Description

    Rabbit polyclonal to ERp29
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-171 of Human ERp29 (Uniprot ID: P30040).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Mouse brain tissue and Human OVCAR3 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
  • Cellular localization

    Endoplasmic reticulum, Cell surface.
  • Database links

  • Alternative names

    • C12orf8 antibody
    • Chromosome 12 open reading frame 8 antibody
    • Endoplasmic reticulum lumenal protein ERp28 antibody
    • Endoplasmic reticulum protein 29 antibody
    • Endoplasmic reticulum protein 29 isoform 1 antibody
    • Endoplasmic reticulum protein ERp29 antibody
    • Endoplasmic reticulum resident protein 28 antibody
    • Endoplasmic reticulum resident protein 29 antibody
    • Endoplasmic reticulum resident protein 31 antibody
    • Epididymis secretory protein Li 107 antibody
    • ERp28 antibody
    • ERP29_HUMAN antibody
    • ERp31 antibody
    • HEL S 107 antibody
    • PDI-DB antibody
    • PDIA9 antibody
    • Protein disulfide isomerase family A member 9 antibody
    see all

Images

  • All lanes : Anti-ERp29 antibody (ab137670) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Anti-ERp29 antibody (ab137670) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells labelling ERp29 (Green) with ab137670 at 1/500 dilution. Alpha-tubulin filaments(Red).
  • Immunohistochemical analysis of paraffin-embedded Human OVCAR3 xenograft tissue labelling ERp29 with ab137670 at 1/500 dilution.

References

ab137670 has not yet been referenced specifically in any publications.

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