Product nameAnti-ERp29 antibody
See all ERp29 primary antibodies
DescriptionChicken polyclonal to ERp29
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human ERp29 aa 33-261 (C terminal).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceProper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Cellular localizationEndoplasmic reticulum, Cell surface.
- C12orf8 antibody
- Chromosome 12 open reading frame 8 antibody
- Endoplasmic reticulum lumenal protein ERp28 antibody
Anti-ERp29 antibody (ab37850) at 1/2000 dilution + E. coli-derived fusion protein.
Goat anti-IgY-HRP at 1/1000
Predicted band size: 29 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding), 40 kDa (possible degradation product), 45 kDa (possible non-specific binding), 45 kDa (possible degradation product), 60 kDa (possible non-specific binding), 60 kDa (possible degradation product)
The actual band size differs from the observed size as this Western blot used a fusion protein as test antigen.
ab37850 has not yet been referenced specifically in any publications.