Anti-ERp29 antibody (ab40982)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-ERp29 antibody
See all ERp29 primary antibodies -
DescriptionRabbit polyclonal to ERp29
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ERp29.
Read Abcam's proprietary immunogen policy (Peptide available as ab42000.) -
Positive control
- This antibody gave a positive signal in the following Human Tissue Lysates: Testis and Liver.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab40982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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RelevanceProper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
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Cellular localizationEndoplasmic reticulum, Cell surface.
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Database links
- Entrez Gene: 613357 Cow
- Entrez Gene: 10961 Human
- Entrez Gene: 67397 Mouse
- Entrez Gene: 117030 Rat
- Omim: 602287 Human
- SwissProt: P81623 Cow
- SwissProt: P30040 Human
- SwissProt: P57759 Mouse
see all -
Alternative names
- C12orf8 antibody
- Chromosome 12 open reading frame 8 antibody
- Endoplasmic reticulum lumenal protein ERp28 antibody
see all
Images
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Western blot - Anti-ERp29 antibody (ab40982)All lanes : Anti-ERp29 antibody (ab40982) at 2 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody (ab40982)IHC image of ERp29 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40982, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Datasheets and documents
References
ab40982 has not yet been referenced specifically in any publications.
