Overview

  • Product name

    Anti-ERp29 antibody [EPR12499(B)]
    See all ERp29 primary antibodies
  • Description

    Rabbit monoclonal [EPR12499(B)] to ERp29
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide within Human ERp29 aa 50-150 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: P30040

  • Positive control

    • HeLa, HepG2, MCF7 and OVCAR-3 cell lysates; HeLa cells; Human kidney and liver tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance

      Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
    • Cellular localization

      Endoplasmic reticulum, Cell surface.
    • Database links

    • Alternative names

      • C12orf8 antibody
      • Chromosome 12 open reading frame 8 antibody
      • Endoplasmic reticulum lumenal protein ERp28 antibody
      • Endoplasmic reticulum protein 29 antibody
      • Endoplasmic reticulum protein 29 isoform 1 antibody
      • Endoplasmic reticulum protein ERp29 antibody
      • Endoplasmic reticulum resident protein 28 antibody
      • Endoplasmic reticulum resident protein 29 antibody
      • Endoplasmic reticulum resident protein 31 antibody
      • Epididymis secretory protein Li 107 antibody
      • ERp28 antibody
      • ERP29_HUMAN antibody
      • ERp31 antibody
      • HEL S 107 antibody
      • PDI-DB antibody
      • PDIA9 antibody
      • Protein disulfide isomerase family A member 9 antibody
      see all

    Images

    • All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : OVCAR-3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 using ab176573 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ERp29 using ab176573 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HeLa cells, labeling ERp29 using ab176573 at a 1/00 dilution (red). DAPI nuclear staining (blue).

    References

    This product has been referenced in:

    • Ye W  et al. ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation. Exp Ther Med 17:817-823 (2019). Read more (PubMed: 30651868) »
    • Chaiyawat P  et al. Endoplasmic reticulum protein 29 (ERp29) as a novel prognostic marker and tumor suppressor in osteosarcoma. J Bone Oncol 16:100233 (2019). Read more (PubMed: 30984555) »
    See all 2 Publications for this product

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