Product nameAnti-ERp29 antibody [EPR12499(B)]
See all ERp29 primary antibodies
DescriptionRabbit monoclonal [EPR12499(B)] to ERp29
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow
Synthetic peptide within Human ERp29 aa 50-150 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: P30040
- HeLa, HepG2, MCF7 and OVCAR-3 cell lysates; HeLa cells; Human kidney and liver tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/100 - 1/250.|
RelevanceProper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Cellular localizationEndoplasmic reticulum, Cell surface.
- C12orf8 antibody
- Chromosome 12 open reading frame 8 antibody
- Endoplasmic reticulum lumenal protein ERp28 antibody
All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : OVCAR-3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 using ab176573 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ERp29 using ab176573 at a 1/50 dilution.
Immunofluorescent analysis of HeLa cells, labeling ERp29 using ab176573 at a 1/00 dilution (red). DAPI nuclear staining (blue).
ab176573 has not yet been referenced specifically in any publications.