Product nameAnti-ERp29 antibody [EPR12985]
See all ERp29 primary antibodies
DescriptionRabbit monoclonal [EPR12985] to ERp29
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ERp29 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P30040
- HepG2, MCF7, A431 and 293T cell lysate; Human kidney and thyroid gland tissues; MCF7 cells
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/50 - 1/100.|
RelevanceProper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Cellular localizationEndoplasmic reticulum, Cell surface.
- C12orf8 antibody
- Chromosome 12 open reading frame 8 antibody
- Endoplasmic reticulum lumenal protein ERp28 antibody
All lanes : Anti-ERp29 antibody [EPR12985] (ab175193) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 with ab175193 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling ERp29 with ab175193 at a 1/50 dilution.
Immunofluorescence analysis of MCF7 cells labeling ERp29 with ab175193 at a 1/50 dilution (red) and DAPI nuclear staining (blue).
ab175193 has not yet been referenced specifically in any publications.