Overview

  • Product name
    Anti-ERp29 antibody [EPR12985]
    See all ERp29 primary antibodies
  • Description
    Rabbit monoclonal [EPR12985] to ERp29
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ERp29 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P30040

  • Positive control
    • HepG2, MCF7, A431 and 293T cell lysate; Human kidney and thyroid gland tissues; MCF7 cells
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
    • Cellular localization
      Endoplasmic reticulum, Cell surface.
    • Database links
    • Alternative names
      • C12orf8 antibody
      • Chromosome 12 open reading frame 8 antibody
      • Endoplasmic reticulum lumenal protein ERp28 antibody
      • Endoplasmic reticulum protein 29 antibody
      • Endoplasmic reticulum protein 29 isoform 1 antibody
      • Endoplasmic reticulum protein ERp29 antibody
      • Endoplasmic reticulum resident protein 28 antibody
      • Endoplasmic reticulum resident protein 29 antibody
      • Endoplasmic reticulum resident protein 31 antibody
      • Epididymis secretory protein Li 107 antibody
      • ERp28 antibody
      • ERP29_HUMAN antibody
      • ERp31 antibody
      • HEL S 107 antibody
      • PDI-DB antibody
      • PDIA9 antibody
      • Protein disulfide isomerase family A member 9 antibody
      see all

    Images

    • All lanes : Anti-ERp29 antibody [EPR12985] (ab175193) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 with ab175193 at a 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling ERp29 with ab175193 at a 1/50 dilution.

    • Immunofluorescence analysis of MCF7 cells labeling ERp29 with ab175193 at a 1/50 dilution (red) and DAPI nuclear staining (blue).

    References

    ab175193 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lung)
    Gel Running Conditions
    Reduced Denaturing (4-15)
    Loading amount
    20 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

    Abcam user community

    Verified customer

    Submitted Dec 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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