Key features and details
- Mouse monoclonal [4F9] to ERp57
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-ERp57 antibody [4F9]
See all ERp57 primary antibodies
DescriptionMouse monoclonal [4F9] to ERp57
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human ERp57 aa 25-505. Full length mature His tagged chain.
Database link: P30101
- WB: HeLa cell lysate. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab223363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 56.8 kDa).|
Tissue specificityDetected in the flagellum and head region of spermatozoa (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERp57 (green) using ab223363 at 1/200 dilution in ICC/IF. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red) at 1/1000 dilution. The lower right panel shows the merged images.
Anti-ERp57 antibody [4F9] (ab223363) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Goat Anti-Mouse IgG HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 56.8 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Block: 5% skimmed milk in TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223363 has not yet been referenced specifically in any publications.