Overview

  • Product name

    Anti-ERp57 antibody [4F9]
    See all ERp57 primary antibodies
  • Description

    Mouse monoclonal [4F9] to ERp57
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human ERp57 aa 25-505. Full length mature His tagged chain.
    Database link: P30101

  • Positive control

    • WB: HeLa cell lysate. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    4F9
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 56.8 kDa).
ICC/IF 1/100.

Target

  • Tissue specificity

    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 58 kDa glucose regulated protein antibody
    • 58 kDa glucose-regulated protein antibody
    • 58 kDa microsomal protein antibody
    • Disulfide isomerase ER 60 antibody
    • Disulfide isomerase ER-60 antibody
    • Endoplasmic reticulum resident protein 57 antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • ER p57 antibody
    • ER protein 57 antibody
    • ER protein 60 antibody
    • ERp 57 antibody
    • ERp57 antibody
    • ERp60 antibody
    • ERp61 antibody
    • Glucose Regulated Protein 58 Kd antibody
    • GRP 57 antibody
    • GRP 58 antibody
    • GRP57 antibody
    • HsT17083 antibody
    • p58 antibody
    • PDIA 3 antibody
    • PDIA3 antibody
    • PDIA3_HUMAN antibody
    • Phospholipase C alpha antibody
    • PI PLC antibody
    • Protein disulfide isomerase A3 antibody
    • Protein disulfide isomerase family A member 3 antibody
    • Protein disulfide-isomerase A3 antibody
    see all

Images

  • 4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERp57 (green) using ab223363 at 1/200 dilution in ICC/IF. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red) at 1/1000 dilution. The lower right panel shows the merged images.

  • Anti-ERp57 antibody [4F9] (ab223363) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 56.8 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Block: 5% skimmed milk in TBST.

References

ab223363 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223363.
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