Anti-ERp57 antibody (ab10287)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERp57 antibody
See all ERp57 primary antibodies -
Description
Rabbit polyclonal to ERp57 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB MouseHuman
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Immunogen
His-tagged E. coli expressed recombinant ERp57.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
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| WB |
Mouse
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa).
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa). |
Target
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Tissue specificity
Detected in the flagellum and head region of spermatozoa (at protein level). -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 2923 Human
- Entrez Gene: 14827 Mouse
- Omim: 602046 Human
- SwissProt: P30101 Human
- SwissProt: P27773 Mouse
- Unigene: 591095 Human
- Unigene: 263177 Mouse
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Alternative names
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
Images
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Western blot - Anti-ERp57 antibody (ab10287)All lanes : Anti-ERp57 antibody (ab10287) at 1/1000 dilution
Lane 1 : Mouse embryonic cells
Lane 2 : Calreticulin deficient mouse embryomic cells
Predicted band size: 61 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?Western blot using ab10287 at 1/1000.
Lane 1 – Mouse Embryonic Fibroblasts.
Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts. -
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)Double staining of mouse embryonic fibroblasts (top) and calreticulin deficient fibroblasts (bottom) with rabbit-anti-ERp57 antibodies (1:100; upper and lower left panel) and FITC-ConA (upper and lower right panel). -
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)This image is courtesy of an anonymous Abreviewab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.
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Western blot - Anti-ERp57 antibody (ab10287)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab10287 has been referenced in 28 publications.
- Bai J et al. Mitochondrial stress-activated cGAS-STING pathway inhibits thermogenic program and contributes to overnutrition-induced obesity in mice. Commun Biol 3:257 (2020). PubMed: 32444826
- Song S et al. Tetrahydrobenzimidazole TMQ0153 triggers apoptosis, autophagy and necroptosis crosstalk in chronic myeloid leukemia. Cell Death Dis 11:109 (2020). PubMed: 32034134
- Bartels AK et al. KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases. Cell Physiol Biochem 52:850-868 (2019). PubMed: 30958660
- Liu CC et al. Integrins and ERp57 Coordinate to Regulate Cell Surface Calreticulin in Immunogenic Cell Death. Front Oncol 9:411 (2019). PubMed: 31192123
- Mazumder A et al. Hydroxycoumarin OT-55 kills CML cells alone or in synergy with imatinib or Synribo: Involvement of ER stress and DAMP release. Cancer Lett 438:197-218 (2018). PubMed: 30205168