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    erp57-antibody-ab10287.pdf

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Signal Transduction Protein Trafficking ER Proteins
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Anti-ERp57 antibody (ab10287)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (28)

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Western blot - Anti-ERp57 antibody (ab10287)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)
  • Western blot - Anti-ERp57 antibody (ab10287)

Key features and details

  • Rabbit polyclonal to ERp57
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ERp57 antibody
    See all ERp57 primary antibodies
  • Description

    Rabbit polyclonal to ERp57
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    His-tagged E. coli expressed recombinant ERp57.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ERp57 protein (ab92937)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
WB
Mouse
Human
Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa).
Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa).

Target

  • Tissue specificity

    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P30101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2923 Human
    • Entrez Gene: 14827 Mouse
    • Omim: 602046 Human
    • SwissProt: P30101 Human
    • SwissProt: P27773 Mouse
    • Unigene: 591095 Human
    • Unigene: 263177 Mouse
    • Alternative names

      • 58 kDa glucose regulated protein antibody
      • 58 kDa glucose-regulated protein antibody
      • 58 kDa microsomal protein antibody
      • Disulfide isomerase ER 60 antibody
      • Disulfide isomerase ER-60 antibody
      • Endoplasmic reticulum resident protein 57 antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • ER p57 antibody
      • ER protein 57 antibody
      • ER protein 60 antibody
      • ERp 57 antibody
      • ERp57 antibody
      • ERp60 antibody
      • ERp61 antibody
      • Glucose Regulated Protein 58 Kd antibody
      • GRP 57 antibody
      • GRP 58 antibody
      • GRP57 antibody
      • HsT17083 antibody
      • p58 antibody
      • PDIA 3 antibody
      • PDIA3 antibody
      • PDIA3_HUMAN antibody
      • Phospholipase C alpha antibody
      • PI PLC antibody
      • Protein disulfide isomerase A3 antibody
      • Protein disulfide isomerase family A member 3 antibody
      • Protein disulfide-isomerase A3 antibody
      see all

    Images

    • Western blot - Anti-ERp57 antibody (ab10287)
      Western blot - Anti-ERp57 antibody (ab10287)
      All lanes : Anti-ERp57 antibody (ab10287) at 1/1000 dilution

      Lane 1 : Mouse embryonic cells
      Lane 2 : Calreticulin deficient mouse embryomic cells

      Predicted band size: 61 kDa
      Observed band size: 57 kDa why is the actual band size different from the predicted?



      Western blot using ab10287 at 1/1000.

      Lane 1 – Mouse Embryonic Fibroblasts.
      Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts.

    • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)
      Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)
      Double staining of mouse embryonic fibroblasts (top) and calreticulin deficient fibroblasts (bottom) with rabbit-anti-ERp57 antibodies (1:100;  upper and lower left panel) and FITC-ConA (upper and lower right panel).
    • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)
      Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)This image is courtesy of an anonymous Abreview

      ab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.

      See Abreview

    • Western blot - Anti-ERp57 antibody (ab10287)
      Western blot - Anti-ERp57 antibody (ab10287)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (28)

    Publishing research using ab10287? Please let us know so that we can cite the reference in this datasheet.

    ab10287 has been referenced in 28 publications.

    • Bai J  et al. Mitochondrial stress-activated cGAS-STING pathway inhibits thermogenic program and contributes to overnutrition-induced obesity in mice. Commun Biol 3:257 (2020). PubMed: 32444826
    • Song S  et al. Tetrahydrobenzimidazole TMQ0153 triggers apoptosis, autophagy and necroptosis crosstalk in chronic myeloid leukemia. Cell Death Dis 11:109 (2020). PubMed: 32034134
    • Bartels AK  et al. KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases. Cell Physiol Biochem 52:850-868 (2019). PubMed: 30958660
    • Liu CC  et al. Integrins and ERp57 Coordinate to Regulate Cell Surface Calreticulin in Immunogenic Cell Death. Front Oncol 9:411 (2019). PubMed: 31192123
    • Mazumder A  et al. Hydroxycoumarin OT-55 kills CML cells alone or in synergy with imatinib or Synribo: Involvement of ER stress and DAMP release. Cancer Lett 438:197-218 (2018). PubMed: 30205168
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-ERp57 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Non-reduced Denaturing (12.5%)
    Sample
    Mouse Tissue lysate - other (white fat tissue)
    Specification
    white fat tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2014

    Question

    Hello,

    Please let me know the below information

    concentration of this antibody

    the dilutions used for IP and for ICC/IF

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 17 2012

    Answer

    Thank you for your enquiry.

    As the datasheet indicates this antibody is whole antiserum and not affinity purified therefore the concentration is not determined. Generally, the IgG concentration for serum antibody is 1-10 mg/ml.

    You may wish to take a look at this site for further information:

    https://www.abcam.com/index.html?pageconfig=technicalfaqs#8

    I would suggest conducting a preliminary experiment and try this antibody at 1:100 and then adjust accordingly from the results obtained.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Dec 17 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Digitonin
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 4% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 14 2007

    Question

    In regard to your polyclonal antibody against ERp57 (ab10287), it is not clear to me whether it was raised against the mouse or human recombinant protein. I would greatly appreciate if you could clarify this to me.

    Read More

    Abcam community

    Verified customer

    Asked on May 13 2004

    Answer

    Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody was raised in rabbit against mouse recombinant ERp57. It does cross-react with mouse but other species have not tested yet.

    Read More

    Abcam Scientific Support

    Answered on May 13 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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