- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-ERp57 antibody
See all ERp57 primary antibodies -
DescriptionRabbit polyclonal to ERp57
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Monkey
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Immunogen
ERp57 from rat liver
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Positive control
- Rat liver microsomal preparation
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab13507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 57 kDa. This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis. | |
| ICC/IF | 1/1000. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 21799787 |
Target
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Tissue specificityDetected in the flagellum and head region of spermatozoa (at protein level).
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Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 281803 Cow
- Entrez Gene: 478279 Dog
- Entrez Gene: 2923 Human
- Entrez Gene: 14827 Mouse
- Entrez Gene: 100156204 Pig
- Entrez Gene: 100328685 Rabbit
- Entrez Gene: 29468 Rat
- Omim: 602046 Human
see all -
Alternative names
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
Images
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Western blot - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen PetrosyanAnti-ERp57 antibody (ab13507) at 1/2000 dilution + Human LNCaP whole cell lysate at 25 µg
Secondary
HRP-conjugated Donkey anti-rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
Exposure time: 45 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyanab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-ERp57 antibody (ab13507)All lanes : Anti-ERp57 antibody (ab13507) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from CHO-K1 cells
Lane 3 : Cell lysates prepared from human liver microsomes
Lane 4 : Cell lysates prepared from rat liver microsomes
Lane 5 : Cell lysates prepared from mouse liver microsomes
Predicted band size: 57 kDa
Protocols
References
This product has been referenced in:
- Lea RG et al. The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals. Sci Rep 6:22279 (2016). IHC . Read more (PubMed: 26931299) »
- Petrosyan A et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). Read more (PubMed: 25605727) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"