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    erp57-antibody-ab13507.pdf

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Signal Transduction Protein Trafficking ER Proteins
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Anti-ERp57 antibody (ab13507)

  • Datasheet
Reviews (6) Submit a question References (9)

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Western blot - Anti-ERp57 antibody (ab13507)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)
  • Western blot - Anti-ERp57 antibody (ab13507)

Key features and details

  • Rabbit polyclonal to ERp57
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Monkey
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human ERp57 protein (ab92937)

View more associated products

Overview

  • Product name

    Anti-ERp57 antibody
    See all ERp57 primary antibodies
  • Description

    Rabbit polyclonal to ERp57
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Monkey
  • Immunogen

    ERp57 from rat liver

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ERp57 protein (ab92937)

Applications

Our Abpromise guarantee covers the use of ab13507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa. This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.
ICC/IF 1/1000.

Target

  • Tissue specificity

    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P30101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281803 Cow
    • Entrez Gene: 478279 Dog
    • Entrez Gene: 2923 Human
    • Entrez Gene: 14827 Mouse
    • Entrez Gene: 100156204 Pig
    • Entrez Gene: 100328685 Rabbit
    • Entrez Gene: 29468 Rat
    • Omim: 602046 Human
    • SwissProt: P38657 Cow
    • SwissProt: P86235 Hamster
    • SwissProt: P30101 Human
    • SwissProt: P27773 Mouse
    • SwissProt: P11598 Rat
    • Unigene: 591095 Human
    • Unigene: 263177 Mouse
    • Unigene: 11527 Rat
    see all
  • Alternative names

    • 58 kDa glucose regulated protein antibody
    • 58 kDa glucose-regulated protein antibody
    • 58 kDa microsomal protein antibody
    • Disulfide isomerase ER 60 antibody
    • Disulfide isomerase ER-60 antibody
    • Endoplasmic reticulum resident protein 57 antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • ER p57 antibody
    • ER protein 57 antibody
    • ER protein 60 antibody
    • ERp 57 antibody
    • ERp57 antibody
    • ERp60 antibody
    • ERp61 antibody
    • Glucose Regulated Protein 58 Kd antibody
    • GRP 57 antibody
    • GRP 58 antibody
    • GRP57 antibody
    • HsT17083 antibody
    • p58 antibody
    • PDIA 3 antibody
    • PDIA3 antibody
    • PDIA3_HUMAN antibody
    • Phospholipase C alpha antibody
    • PI PLC antibody
    • Protein disulfide isomerase A3 antibody
    • Protein disulfide isomerase family A member 3 antibody
    • Protein disulfide-isomerase A3 antibody
    see all

Images

  • Western blot - Anti-ERp57 antibody (ab13507)
    Western blot - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyan
    Anti-ERp57 antibody (ab13507) at 1/2000 dilution + Human LNCaP whole cell lysate at 25 µg

    Secondary
    HRP-conjugated Donkey anti-rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 45 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyan

    ab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)
    ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-ERp57 antibody (ab13507)
    Western blot - Anti-ERp57 antibody (ab13507)
    All lanes : Anti-ERp57 antibody (ab13507) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from CHO-K1 cells
    Lane 3 : Cell lysates prepared from human liver microsomes
    Lane 4 : Cell lysates prepared from rat liver microsomes
    Lane 5 : Cell lysates prepared from mouse liver microsomes

    Predicted band size: 57 kDa

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (9)

    Publishing research using ab13507? Please let us know so that we can cite the reference in this datasheet.

    ab13507 has been referenced in 9 publications.

    • Lea RG  et al. The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals. Sci Rep 6:22279 (2016). IHC . PubMed: 26931299
    • Petrosyan A  et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). PubMed: 25605727
    • Zhao Q  et al. Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis. Proteome Sci 13:18 (2015). WB . PubMed: 26155198
    • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). PubMed: 25086069
    • Li J  et al. Identification and characterization of an oocyte factor required for sperm decondensation in pig. Reproduction 148:367-75 (2014). PubMed: 25030891
    • Al-Gubory KH  et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction 147:599-614 (2014). WB, IHC-P ; Sheep . PubMed: 24446454
    • Yu Y  et al. Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice. PLoS One 6:e22172 (2011). IHC-P, WB ; Mouse . PubMed: 21799787
    • Ding Y  et al. Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome. Proteomics 10:2620-2630 (2010). WB ; Rat . PubMed: 20461717
    • Atkin JD  et al. Induction of the unfolded protein response in familial amyotrophic lateral sclerosis and association of protein-disulfide isomerase with superoxide dismutase 1. J Biol Chem 281:30152-65 (2006). PubMed: 16847061

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice Hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice Hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Apr 04 2019

    Western blot abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Feb 15 2019

    Western blot abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - other (Rat hepatocytes - Golgi fraction)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    20 µg
    Specification
    Rat hepatocytes - Golgi fraction
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 19 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Rat Cell (Rat hepatocytes)
    Specification
    Rat hepatocytes
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Oct 29 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Human Cell (PC-3 - prostate cancer cells)
    Specification
    PC-3 - prostate cancer cells
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 13 2013

    Western blot abreview for Anti-ERp57 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Sample
    Human Cell lysate - whole cell (LNCaP)
    Specification
    LNCaP
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 13 2013

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