Key features and details
- Goat polyclonal to ERp57
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ERp57 antibody
See all ERp57 primary antibodies
DescriptionGoat polyclonal to ERp57
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Dog, Monkey
Recombinant fragment within Human ERp57 aa 300-505. The exact sequence is proprietary.
Database link: P30101
- Human kidney tissue; RPE cells; Hepa and TR-iBRB cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|WB||1/500 - 1/2000. Predicted molecular weight: 57 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityDetected in the flagellum and head region of spermatozoa (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
All lanes : Anti-ERp57 antibody (ab219393) at 1/500 dilution
Lane 1 : Hepa cell lysates
Lane 2 : TR-iBRB cell lysates
Lysates/proteins at 100 µg per lane.
All lanes : Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 57 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling ERp57 using ab219393 at 5 µg/ml.
Immunofluorescence analysis of primary RPE cells labeling ERp57 using ab219393 at 1/100 dilution. Cells were fixed with 4% of PFA.
ab219393 has not yet been referenced specifically in any publications.