Overview

  • Product name

  • Description

    Rabbit polyclonal to ERp57
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ERp57 (internal sequence). The exact sequence is proprietary.
    Database link: P30101

  • Positive control

    • WB: HepG2, HCT 116, Neuro-2a, GL261 and C8D30 whole cell lysates; HEK-293T, A431, HeLa, PC-12 and Rat2 whole cell extracts. IHC-P: U87 xenograft tissue; Mouse testis tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity

    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 58 kDa glucose regulated protein antibody
    • 58 kDa glucose-regulated protein antibody
    • 58 kDa microsomal protein antibody
    • Disulfide isomerase ER 60 antibody
    • Disulfide isomerase ER-60 antibody
    • Endoplasmic reticulum resident protein 57 antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • ER p57 antibody
    • ER protein 57 antibody
    • ER protein 60 antibody
    • ERp 57 antibody
    • ERp57 antibody
    • ERp60 antibody
    • ERp61 antibody
    • Glucose Regulated Protein 58 Kd antibody
    • GRP 57 antibody
    • GRP 58 antibody
    • GRP57 antibody
    • HsT17083 antibody
    • p58 antibody
    • PDIA 3 antibody
    • PDIA3 antibody
    • PDIA3_HUMAN antibody
    • Phospholipase C alpha antibody
    • PI PLC antibody
    • Protein disulfide isomerase A3 antibody
    • Protein disulfide isomerase family A member 3 antibody
    • Protein disulfide-isomerase A3 antibody
    see all

Images

  • All lanes : Anti-ERp57 antibody (ab228789) at 1/500 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERp57 (green) using ab228789 at 1/1000 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue). alpha Tubulin, a cytoskeleton marker, was stained by an anti-alpha Tubulin antibody at 1/1000 dilution.

  • Paraffin-embedded mouse testis tissue stained for ERp57 using ab228789 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded U87 (human) xenograft tissue stained for ERp57 using ab228789 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-ERp57 antibody (ab228789) at 1/10000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-ERp57 antibody (ab228789) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-ERp57 antibody (ab228789) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS-PAGE gel.

References

ab228789 has not yet been referenced specifically in any publications.

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