Product nameAnti-ERp57 antibody - C-terminal
See all ERp57 primary antibodies
DescriptionGoat polyclonal to ERp57 - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Monkey
Recombinant fragment within Human ERp57 aa 300-505 (C terminal). The exact sequence is proprietary. Produced in E. coli.
Database link: P30101
- MNT1 and RAW 264.7 lysates; RPE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 20% Glycerol, 79% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|WB||1/500 - 1/2000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/200 - 1/1000.|
|IHC-Fr||1/200 - 1/1000.|
Tissue specificityDetected in the flagellum and head region of spermatozoa (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
All lanes : Anti-ERp57 antibody - C-terminal (ab192444) at 1/500 dilution
Lane 1 : MNT1 lysate
Lane 2 : RAW 264.7 lysate
Lysates/proteins at 100 µg per lane.
All lanes : rabbit anti-goat HRP at 1/10000 dilution
Predicted band size: 57 kDa
Immunofluorescent analysis of RPE cells (4% PFA-fixed) labeling ERp57 with ab192444 at 1/100 dilution.
ab192444 has not yet been referenced specifically in any publications.