Key features and details
- Goat polyclonal to ERp57 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Dog, Human, Monkey
- Isotype: IgG
Product nameAnti-ERp57 antibody - C-terminal
See all ERp57 primary antibodies
DescriptionGoat polyclonal to ERp57 - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Monkey
Recombinant fragment within Human ERp57 aa 300-505 (C terminal). The exact sequence is proprietary. Produced in E. coli.
Database link: P30101
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 20% Glycerol, 79% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|WB||1/500 - 1/2000. Predicted molecular weight: 57 kDa.|
Tissue specificityDetected in the flagellum and head region of spermatozoa (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
All lanes : Anti-ERp57 antibody - C-terminal (ab192444) at 1/500 dilution
Lane 1 : MNT1 lysate
Lane 2 : RAW 264.7 lysate
Lysates/proteins at 100 µg per lane.
All lanes : rabbit anti-goat HRP at 1/10000 dilution
Predicted band size: 57 kDa
Immunofluorescent analysis of RPE cells (4% PFA-fixed) labeling ERp57 with ab192444 at 1/100 dilution.
ab192444 has not yet been referenced specifically in any publications.