Overview

  • Product name

    Anti-ERp57 antibody - C-terminal
    See all ERp57 primary antibodies
  • Description

    Goat polyclonal to ERp57 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant fragment within Human ERp57 aa 300-505 (C terminal). The exact sequence is proprietary. Produced in E. coli.
    Database link: P30101

  • Positive control

    • MNT1 and RAW 264.7 lysates; RPE cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 20% Glycerol, 79% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
WB 1/500 - 1/2000. Predicted molecular weight: 57 kDa.
IHC-P 1/200 - 1/1000.
IHC-Fr 1/200 - 1/1000.

Target

  • Tissue specificity

    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 58 kDa glucose regulated protein antibody
    • 58 kDa glucose-regulated protein antibody
    • 58 kDa microsomal protein antibody
    • Disulfide isomerase ER 60 antibody
    • Disulfide isomerase ER-60 antibody
    • Endoplasmic reticulum resident protein 57 antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • ER p57 antibody
    • ER protein 57 antibody
    • ER protein 60 antibody
    • ERp 57 antibody
    • ERp57 antibody
    • ERp60 antibody
    • ERp61 antibody
    • Glucose Regulated Protein 58 Kd antibody
    • GRP 57 antibody
    • GRP 58 antibody
    • GRP57 antibody
    • HsT17083 antibody
    • p58 antibody
    • PDIA 3 antibody
    • PDIA3 antibody
    • PDIA3_HUMAN antibody
    • Phospholipase C alpha antibody
    • PI PLC antibody
    • Protein disulfide isomerase A3 antibody
    • Protein disulfide isomerase family A member 3 antibody
    • Protein disulfide-isomerase A3 antibody
    see all

Images

  • All lanes : Anti-ERp57 antibody - C-terminal (ab192444) at 1/500 dilution

    Lane 1 : MNT1 lysate
    Lane 2 : RAW 264.7 lysate

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : rabbit anti-goat HRP at 1/10000 dilution

    Predicted band size: 57 kDa

  • Immunofluorescent analysis of RPE cells (4% PFA-fixed) labeling ERp57 with ab192444 at 1/100 dilution.

References

ab192444 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192444.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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