Overview

  • Product name
    Anti-ERp72 antibody
    See all ERp72 primary antibodies
  • Description
    Rabbit polyclonal to ERp72
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Guinea pig, Hamster, Cow, Dog, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 623-638 of Mouse ERp72, with a cysteine residue added.

  • Positive control
    • WB: liver microsome extracts. IHC-P: Human cerebellum, placenta, testis or prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab109869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 73 kDa).
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Calcium binding protein intestinal related antibody
    • Endoplasmic reticulum resident protein 70 antibody
    • Endoplasmic reticulum resident protein 72 antibody
    • EPR70 antibody
    • EPR72 antibody
    • ER protein 70 antibody
    • ER protein 72 antibody
    • ERP 70 antibody
    • ERp 72 antibody
    • ERp-72 antibody
    • ERp70 antibody
    • ERp72 antibody
    • PDIA 4 antibody
    • Pdia4 antibody
    • PDIA4_HUMAN antibody
    • PDIR antibody
    • Protein disulfide isomerase A4 antibody
    • Protein disulfide isomerase associated 4 antibody
    • Protein disulfide isomerase family A member 4 antibody
    • protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A4 antibody
    • Protein ERp 72 antibody
    • Protein ERp72 antibody
    see all

Images

  • Western blot - Anti-ERp72 antibody (ab109869)
    Western blot - Anti-ERp72 antibody (ab109869)
    All lanes : Anti-ERp72 antibody (ab109869) at 1/1000 dilution

    Lane 1 : Rat liver microsome extracts
    Lane 2 : Mouse liver microsome extracts
    Lane 3 : Human liver microsome extracts

    Predicted band size: 73 kDa
    Observed band size: 72 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    ab109869 at 10 µg/ml staining ERp72 in formalin-fixed, paraffin-embedded Human cerebellum tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded Human placenta tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)
    ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded Human testis tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab109869 has not yet been referenced specifically in any publications.

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