Anti-ERp72 antibody (ab109869)
Key features and details
- Rabbit polyclonal to ERp72
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ERp72 antibody
See all ERp72 primary antibodies -
Description
Rabbit polyclonal to ERp72 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Guinea pig, Hamster, Cow, Dog, Monkey
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Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 623-638 of Mouse ERp72, with a cysteine residue added.
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Positive control
- WB: liver microsome extracts. IHC-P: Human cerebellum, placenta, testis or prostate tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab109869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 73 kDa). | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 415110 Cow
- Entrez Gene: 9601 Human
- Entrez Gene: 12304 Mouse
- Entrez Gene: 116598 Rat
- SwissProt: Q29RV1 Cow
- SwissProt: P13667 Human
- SwissProt: P08003 Mouse
- SwissProt: P38659 Rat
see all -
Alternative names
- Calcium binding protein intestinal related antibody
- Endoplasmic reticulum resident protein 70 antibody
- Endoplasmic reticulum resident protein 72 antibody
see all
Images
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Western blot - Anti-ERp72 antibody (ab109869)All lanes : Anti-ERp72 antibody (ab109869) at 1/1000 dilution
Lane 1 : Rat liver microsome extracts
Lane 2 : Mouse liver microsome extracts
Lane 3 : Human liver microsome extracts
Predicted band size: 73 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining ERp72 in formalin-fixed, paraffin-embedded Human cerebellum tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded Human placenta tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded Human testis tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
References (0)
ab109869 has not yet been referenced specifically in any publications.
