Anti-ERp72 antibody (ab109869)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ERp72 antibody
See all ERp72 primary antibodies -
Description
Rabbit polyclonal to ERp72 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Guinea pig, Hamster, Cow, Dog, Monkey
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Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 623-638 of Mouse ERp72, with a cysteine residue added.
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Positive control
- WB: liver microsome extracts. IHC-P: Human cerebellum, placenta, testis or prostate tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab109869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 73 kDa). | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 415110 Cow
- Entrez Gene: 9601 Human
- Entrez Gene: 12304 Mouse
- Entrez Gene: 116598 Rat
- SwissProt: Q29RV1 Cow
- SwissProt: P13667 Human
- SwissProt: P08003 Mouse
- SwissProt: P38659 Rat
see all -
Alternative names
- Calcium binding protein intestinal related antibody
- Endoplasmic reticulum resident protein 70 antibody
- Endoplasmic reticulum resident protein 72 antibody
see all
Images
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Western blot - Anti-ERp72 antibody (ab109869)All lanes : Anti-ERp72 antibody (ab109869) at 1/1000 dilution
Lane 1 : Rat liver microsome extracts
Lane 2 : Mouse liver microsome extracts
Lane 3 : Human liver microsome extracts
Predicted band size: 73 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining ERp72 in formalin-fixed, paraffin-embedded Human cerebellum tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded Human placenta tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab109869)ab109869 at 10 µg/ml staining Erp72 in formalin-fixed, paraffin-embedded Human testis tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
References
ab109869 has not yet been referenced specifically in any publications.
