- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-ERp72 antibody
See all ERp72 primary antibodies -
DescriptionRabbit polyclonal to ERp72
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 393-645 of Human ERp72 (Uniprot ID P13667)
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Positive control
- 293T, A431, NIH-3T3, JC, BCL-1 whole cell lysates, methanol-fixed HeLa cells, paraffin-embedded Breast carcinoma
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 73 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 415110 Cow
- Entrez Gene: 9601 Human
- Entrez Gene: 12304 Mouse
- Entrez Gene: 116598 Rat
- SwissProt: Q29RV1 Cow
- SwissProt: P13667 Human
- SwissProt: P08003 Mouse
- SwissProt: P38659 Rat
see all -
Alternative names
- Calcium binding protein intestinal related antibody
- Endoplasmic reticulum resident protein 70 antibody
- Endoplasmic reticulum resident protein 72 antibody
see all
Images
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Western blot - Anti-ERp72 antibody (ab155800)All lanes : Anti-ERp72 antibody (ab155800) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
7.5% SDS PAGE -
Western blot - Anti-ERp72 antibody (ab155800)All lanes : Anti-ERp72 antibody (ab155800) at 1/10000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ERp72 antibody (ab155800)Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling ERp72 with ab155800 at 1/500 dilution (green). Bottom image shows merger with nuclear staining (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab155800)Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling ERp72with ab155800 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Paliwal P & Conboy IM Inhibitors of tyrosine phosphatases and apoptosis reprogram lineage-marked differentiated muscle to myogenic progenitor cells. Chem Biol 18:1153-66 (2011). Read more (PubMed: 21944754) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"