Overview

  • Product name
  • Description
    Rabbit polyclonal to ERp72
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 393-645 of Human ERp72 (Uniprot ID P13667)

  • Positive control
    • 293T, A431, NIH-3T3, JC, BCL-1 whole cell lysates, methanol-fixed HeLa cells, paraffin-embedded Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab155800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium binding protein intestinal related antibody
    • Endoplasmic reticulum resident protein 70 antibody
    • Endoplasmic reticulum resident protein 72 antibody
    • EPR70 antibody
    • EPR72 antibody
    • ER protein 70 antibody
    • ER protein 72 antibody
    • ERP 70 antibody
    • ERp 72 antibody
    • ERp-72 antibody
    • ERp70 antibody
    • ERp72 antibody
    • PDIA 4 antibody
    • Pdia4 antibody
    • PDIA4_HUMAN antibody
    • PDIR antibody
    • Protein disulfide isomerase A4 antibody
    • Protein disulfide isomerase associated 4 antibody
    • Protein disulfide isomerase family A member 4 antibody
    • protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A4 antibody
    • Protein ERp 72 antibody
    • Protein ERp72 antibody
    see all

Images

  • All lanes : Anti-ERp72 antibody (ab155800) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • All lanes : Anti-ERp72 antibody (ab155800) at 1/10000 dilution

    Lane 1 : NIH-3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling ERp72 with ab155800 at 1/500 dilution (green). Bottom image shows merger with nuclear staining (blue).
  • Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling ERp72with ab155800 at 1/500 dilution.

References

This product has been referenced in:
  • Paliwal P & Conboy IM Inhibitors of tyrosine phosphatases and apoptosis reprogram lineage-marked differentiated muscle to myogenic progenitor cells. Chem Biol 18:1153-66 (2011). Read more (PubMed: 21944754) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

The HeLa ICC/IF on the datasheet was produced as follows.

1. Culture cells on coverslips. (Chamber slides are an alternative).

2. Fix with 500 ul pre-cooled methanol, -20C, for 5 minutes.

3. Wash with PBS twice, 3 minutes per wash.

4. Block non-specific protein interactions with 500 ul of 2.5% BSA/PBS for 30 minutes.

5. Remove blocking solution (washing is not necessary) and add the Erp72 antibody, 200 - 400 ul, diluted 1/500 in 2.5% BSA/PBS. Incubate for 1 hour at RT or 4C overnight (recommended).

6. Wash 3X with PBS for 3 minutes per wash.

7. Add 200 - 400 ul secondary anti-rabbit Igg, conjugated to the fluorchrome of choice, for instance, ab150073,

Click here (or use the following: https://www.abcam.com/index.html?datasheet=150073).

8. Incubate at RT for 60 minutes.

9. Wash 3X with PBS for 3 minutes per wash.

10. Counterstain if desired (e.g., DAPI nuclear counterstain), and mount coverslips with anti-fading mounting medium, for instance, ab104135 or ab104139 (contains DAPI, so there is no need to counterstain beforehand).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=104135).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=104139).

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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