Anti-ERp72 antibody (ab155800)

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Overview

  • Product name

    Anti-ERp72 antibody
    See all ERp72 primary antibodies

  • Description

    Rabbit polyclonal to ERp72

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 393-645 of Human ERp72 (Uniprot ID P13667)

  • Positive control

    • 293T, A431, NIH-3T3, JC, BCL-1 whole cell lysates, methanol-fixed HeLa cells, paraffin-embedded Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab155800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.

  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Calcium binding protein intestinal related antibody
    • Endoplasmic reticulum resident protein 70 antibody
    • Endoplasmic reticulum resident protein 72 antibody
    • EPR70 antibody
    • EPR72 antibody
    • ER protein 70 antibody
    • ER protein 72 antibody
    • ERP 70 antibody
    • ERp 72 antibody
    • ERp-72 antibody
    • ERp70 antibody
    • ERp72 antibody
    • PDIA 4 antibody
    • Pdia4 antibody
    • PDIA4_HUMAN antibody
    • PDIR antibody
    • Protein disulfide isomerase A4 antibody
    • Protein disulfide isomerase associated 4 antibody
    • Protein disulfide isomerase family A member 4 antibody
    • protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A4 antibody
    • Protein ERp 72 antibody
    • Protein ERp72 antibody
    see all

Images

  • Western blot - Anti-ERp72 antibody (ab155800)
    Western blot - Anti-ERp72 antibody (ab155800)
    All lanes : Anti-ERp72 antibody (ab155800) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Western blot - Anti-ERp72 antibody (ab155800)
    Western blot - Anti-ERp72 antibody (ab155800)
    All lanes : Anti-ERp72 antibody (ab155800) at 1/10000 dilution

    Lane 1 : NIH-3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp72 antibody (ab155800)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp72 antibody (ab155800)
    Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling ERp72 with ab155800 at 1/500 dilution (green). Bottom image shows merger with nuclear staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab155800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab155800)
    Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling ERp72with ab155800 at 1/500 dilution.

References

This product has been referenced in:

See all 5 Publications for this product

Publishing research using ab155800? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

The immunofluorescence image for the ERp72 antibody, is there any additional information that you could send on the antibody, the procedure and etc, which helped produce that image? Secondly, do you know of any publications using this antibody in immunofluorescence?

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Answer

The HeLa ICC/IF on the datasheet was produced as follows.

1. Culture cells on coverslips. (Chamber slides are an alternative).

2. Fix with 500 ul pre-cooled methanol, -20C, for 5 minutes.

3. Wash with PBS twice, 3 minutes per wash.

4. Block non-specific protein interactions with 500 ul of 2.5% BSA/PBS for 30 minutes.

5. Remove blocking solution (washing is not necessary) and add the Erp72 antibody, 200 - 400 ul, diluted 1/500 in 2.5% BSA/PBS. Incubate for 1 hour at RT or 4C overnight (recommended).

6. Wash 3X with PBS for 3 minutes per wash.

7. Add 200 - 400 ul secondary anti-rabbit Igg, conjugated to the fluorchrome of choice, for instance, ab150073,

Click here (or use the following: https://www.abcam.com/index.html?datasheet=150073).

8. Incubate at RT for 60 minutes.

9. Wash 3X with PBS for 3 minutes per wash.

10. Counterstain if desired (e.g., DAPI nuclear counterstain), and mount coverslips with anti-fading mounting medium, for instance, ab104135 or ab104139 (contains DAPI, so there is no need to counterstain beforehand).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=104135).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=104139).

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