Anti-ERp72 antibody (ab155800)
Key features and details
- Rabbit polyclonal to ERp72
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERp72 antibody
See all ERp72 primary antibodies -
Description
Rabbit polyclonal to ERp72 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 393-645 of Human ERp72 (Uniprot ID P13667)
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Positive control
- 293T, A431, NIH-3T3, JC, BCL-1 whole cell lysates, methanol-fixed HeLa cells, paraffin-embedded Breast carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 73 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used |
ICC/IF
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 415110 Cow
- Entrez Gene: 9601 Human
- Entrez Gene: 12304 Mouse
- Entrez Gene: 116598 Rat
- SwissProt: Q29RV1 Cow
- SwissProt: P13667 Human
- SwissProt: P08003 Mouse
- SwissProt: P38659 Rat
see all -
Alternative names
- Calcium binding protein intestinal related antibody
- Endoplasmic reticulum resident protein 70 antibody
- Endoplasmic reticulum resident protein 72 antibody
see all
Images
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All lanes : Anti-ERp72 antibody (ab155800) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
7.5% SDS PAGE -
All lanes : Anti-ERp72 antibody (ab155800) at 1/10000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
7.5% SDS PAGE -
Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling ERp72 with ab155800 at 1/500 dilution (green). Bottom image shows merger with nuclear staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (ab155800)Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling ERp72with ab155800 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab155800 has been referenced in 5 publications.
- Yin F et al. Microarray-based identification of genes associated with prognosis and drug resistance in ovarian cancer. J Cell Biochem 120:6057-6070 (2019). PubMed: 30335894
- Chia J et al. The GalNAc-T Activation (GALA) Pathway: Drivers and markers. PLoS One 14:e0214118 (2019). PubMed: 30889231
- Tsuchiya Y et al. IRE1-XBP1 pathway regulates oxidative proinsulin folding in pancreatic ß cells. J Cell Biol 217:1287-1301 (2018). PubMed: 29507125
- Nguyen AT et al. Organelle Specific O-Glycosylation Drives MMP14 Activation, Tumor Growth, and Metastasis. Cancer Cell 32:639-653.e6 (2017). PubMed: 29136507
- Paliwal P & Conboy IM Inhibitors of tyrosine phosphatases and apoptosis reprogram lineage-marked differentiated muscle to myogenic progenitor cells. Chem Biol 18:1153-66 (2011). PubMed: 21944754