Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14824(B)] to ERp72
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-ERp72 antibody [EPR14824(B)]
See all ERp72 primary antibodies
DescriptionRabbit monoclonal [EPR14824(B)] to ERp72
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ERp72 aa 100-200. The exact sequence is proprietary.
Database link: P13667
- HeLa, JAR and MCF7 cell lysates; Human fetal liver lysate. Human lung carcinoma tissue. MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Calcium binding protein intestinal related antibody
- Endoplasmic reticulum resident protein 70 antibody
- Endoplasmic reticulum resident protein 72 antibody
All lanes : Anti-ERp72 antibody [EPR14824(B)] (ab190348) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Immunohistochemical analysis of paraffin-embedded, Human lung carcinoma tissue labeling ERp72 with ab190348 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow cytometry analysis of 2% paraformaldehyde-fixed MCF7 cells labeling ERp72 with ab190348 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
ab190348 has not yet been referenced specifically in any publications.