Anti-ESAM antibody (ab74777)
Key features and details
- Rabbit polyclonal to ESAM
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ESAM antibody -
Description
Rabbit polyclonal to ESAM -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human ESAM aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101730) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Heart tissue; Human Liver tissue; Human Kidney tissue; Human Lung tissue; HepG2 whole cell; HEK293 whole cell.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Can mediate aggregation most likely through a homophilic molecular interaction. -
Tissue specificity
Highly expressed in endothelial cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell junction > adherens junction. Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 90952 Human
- Omim: 614281 Human
- SwissProt: Q96AP7 Human
- Unigene: 173840 Human
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Alternative names
- 2310008D05Rik antibody
- Endothelial cell adhesion molecule antibody
- Endothelial cell selective adhesion molecule antibody
see all
Images
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All lanes : Anti-ESAM antibody (ab74777) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (ab94080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 37,41 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 15 kDa, 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The ESAM protein has a predicted molecular weight of 41 kDa. The first 29 amino acids of the ESAM sequence act as a signal sequence. The band at 37 kDa may represent the cleaved form of the ESAM protein.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab74777 has not yet been referenced specifically in any publications.