Product nameAnti-ESAM antibody
See all ESAM primary antibodies
DescriptionRabbit polyclonal to ESAM
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
Synthetic peptide corresponding to Human ESAM aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Human Heart tissue; Human Liver tissue; Human Kidney tissue; Human Lung tissue; HepG2 whole cell; HEK293 whole cell.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).|
FunctionCan mediate aggregation most likely through a homophilic molecular interaction.
Tissue specificityHighly expressed in endothelial cells.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell junction > adherens junction. Cell junction > tight junction. Cell membrane.
- Information by UniProt
- 2310008D05Rik antibody
- Endothelial cell adhesion molecule antibody
- Endothelial cell selective adhesion molecule antibody
All lanes : Anti-ESAM antibody (ab74777) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Annexin-2/ANXA2 overexpression lysate (ab94080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 37,41 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 15 kDa, 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The ESAM protein has a predicted molecular weight of 41 kDa. The first 29 amino acids of the ESAM sequence act as a signal sequence. The band at 37 kDa may represent the cleaved form of the ESAM protein.
ICC/IF image of ab74777 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74777, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.
ab74777 has not yet been referenced specifically in any publications.