Anti-ESAM antibody (ab74777)


  • Product name
  • Description
    Rabbit polyclonal to ESAM
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human ESAM.

    (Peptide available as ab101730.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Heart tissue; Human Liver tissue; Human Kidney tissue; Human Lung tissue; HepG2 whole cell; HEK293 whole cell.



Our Abpromise guarantee covers the use of ab74777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).


  • Function
    Can mediate aggregation most likely through a homophilic molecular interaction.
  • Tissue specificity
    Highly expressed in endothelial cells.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell junction > adherens junction. Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310008D05Rik antibody
    • Endothelial cell adhesion molecule antibody
    • Endothelial cell selective adhesion molecule antibody
    • Endothelial cell-selective adhesion molecule antibody
    • Esam antibody
    • ESAM_HUMAN antibody
    • HUEL (C4orf1) interacting protein antibody
    • LP4791 protein antibody
    • W117m antibody
    see all


  • All lanes : Anti-ESAM antibody (ab74777) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Annexin A2 overexpression 293T lysate (whole cell) (ab94080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 37,41 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 100 kDa, 15 kDa, 250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes

    The ESAM protein has a predicted molecular weight of 41 kDa. The first 29 amino acids of the ESAM sequence act as a signal sequence. The band at 37 kDa may represent the cleaved form of the ESAM protein.
  • ICC/IF image of ab74777 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74777, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.


ab74777 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Sorry I was not able to take your email last week. I was not in the office. I am very sorry that the two anti-ESAM antibodies did not show the staining we had hoped for, it may be that as you say that the epitope presented by the protein (even thou...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Colon tumour)
Colon tumour
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 08 2011

Thank you for contacting us. We currently have two antibodies directed towards human EMSA, ab74777 and ab73988. Both are rabbit polyclonals. ab74777 like the antibody you have previously used is directed against the extracellular domain of ESAM...

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