Overview

  • Product name
    Anti-ESAT6 antibody [11G4]
    See all ESAT6 primary antibodies
  • Description
    Mouse monoclonal [11G4] to ESAT6
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium szulgai and Mycobacterium marinum.
  • Immunogen

    Tissue/ cell preparation: PPD from Mycobacterium tuberculosis (Tuberculin PPD).

  • Epitope
    This antibody binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 of ESAT6.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purification notes
    Protein A/G purified.
  • Clonality
    Monoclonal
  • Clone number
    11G4
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa.

Detects a band of approximately 10 kDa in short term culture of M. tuberculosis.

ELISA 1/3200.

Target

  • Relevance
    The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
  • Cellular localization
    Secreted
  • Alternative names
    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

Images

  • All lanes : Anti-ESAT6 antibody [11G4] (ab26246) at 1/5000 dilution

    All lanes : M. tuberculosis culture filtrate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-mouse monoclonal at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 11 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocked with 5% milk for 1 hour at 22°C

    See Abreview

References

This product has been referenced in:
  • Aguilo N  et al. Reactogenicity to major tuberculosis antigens absent in BCG is linked to improved protection against Mycobacterium tuberculosis. Nat Commun 8:16085 (2017). Read more (PubMed: 28706226) »
  • Sevastsyanovich YR  et al. Antigen Localization Influences the Magnitude and Kinetics of Endogenous Adaptive Immune Response to Recombinant Salmonella Vaccines. Infect Immun 85:N/A (2017). WB . Read more (PubMed: 28893919) »
See all 17 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mycobacterium tuberculosis Cell lysate - other (M. tuerculosis culture filtrate)
Total protein in input
1000 µg
Specification
M. tuerculosis culture filtrate
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Jul 11 2012

Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
10 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 27 2012

Answer

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding sequences of these have unfortunately not been determined, except for ab26246 (residues EQQWNFAGIEAAA at positions 3-15). So if you are working with protein fragments (or epitopes), we cannot guarantee that these antibodies will work for you, as the binding sites might not be present and therefore the antibody might not bind. I could ask the lab whether the epitopes have been mapped and whether the antibodies would bind your fragments, if you could provide some details to your peptides.

2) We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service:

United States

Pocono Rabbit Farm and Laboratory (PRFL)
PO Box 240
Canadensis, PA 18325
Tel: 800-622-6381 - Toll Free in the United Statesand Canada
Tel: 570-595-7108 - Worldwide
Fax: 570-595-9277
Website: www.prfal.com

Maine Biotechnology Services, Inc.
1037R Forest Ave.
Portland Maine 04103
Tel: 800-925-9476 or 207-797-5454
Fax: 207-797-5595
Website: www.mainebiotechnology.com

Cocalico Biologicals, Inc.
PO Box 265
449 Stevens Road
Reamstown, PA 17567-0265
Tel: 717-336-1990
Fax: 717-336-1993
Website: www.cocalicobiologicals.com

United Kingdom

Charles River Laboratories International, Inc.
Tel: +44 (0) 1843 823575
Website: www.criver.com

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

Read More

Answer

Thank you for contacting us. The optimal dilution will depend on the nature and amount of your sample but I recommend starting with a dilution of 1/500 (2 ug/ml). Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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