Overview

  • Product name

  • Description

    Rabbit polyclonal to ESAT6
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with recombinant ESAT6 produced in E.coli.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein (Rv3875) from Mycobacterium tubercolosis.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). Use under reducing conditions.

Target

  • Relevance

    The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
  • Cellular localization

    Secreted
  • Alternative names

    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

References

This product has been referenced in:

  • Sepulveda D  et al. Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem. Sensors (Basel) 17:N/A (2017). Read more (PubMed: 28937645) »
  • Tan K  et al. Comparison of BCG prime-DNA booster and rBCG regimens for protection against tuberculosis. Hum Vaccin Immunother 10:391-8 (2014). Read more (PubMed: 24192709) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mycobacterium tuberculosis Recombinant protein (Cell lysate, supernatant)
Specification
Cell lysate, supernatant
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 18 2014

Application
Immunoprecipitation
Sample
Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate)
Total protein in input
1000 µg
Specification
M. tuberculosis culture filtrate
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Jul 11 2012

Answer

Thank you for contacting us.
Your credit note ID is ***.
I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only. Please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Answer

Thank you very much for your reply.

I can certainly arrange a credit or refund for you for this purchase. A credit can be held on your account and used against a future order, or we can issue a full refund. Do you have any information about your order, such as the order number or PO number? If this was ordered through a distributor, could you tell me which company you ordered throughand the date of the purchase?

I look forward to hearing from you. Please let me know if you have further questions, and have a nice day.

Read More

Answer

Thank you for submitting your recent review of ab45073. I am sorry to see that the results with this antibody were so poor.

We do fully guarantee this antibody to work in Western blot with M tuberculosis samples, so if you would like, I can send you a different antibody as a replacement for this product. I can alternatively arrange a credit or refund if you would prefer. If you'd like to accept one of these options, please let me know your original order information and I will set this up for you.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More
Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
15 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jul 02 2012

Answer

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding sequences of these have unfortunately not been determined, except for ab26246 (residues EQQWNFAGIEAAA at positions 3-15). So if you are working with protein fragments (or epitopes), we cannot guarantee that these antibodies will work for you, as the binding sites might not be present and therefore the antibody might not bind. I could ask the lab whether the epitopes have been mapped and whether the antibodies would bind your fragments, if you could provide some details to your peptides.

2) We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service:

United States

Pocono Rabbit Farm and Laboratory (PRFL)
PO Box 240
Canadensis, PA 18325
Tel: 800-622-6381 - Toll Free in the United Statesand Canada
Tel: 570-595-7108 - Worldwide
Fax: 570-595-9277
Website: www.prfal.com

Maine Biotechnology Services, Inc.
1037R Forest Ave.
Portland Maine 04103
Tel: 800-925-9476 or 207-797-5454
Fax: 207-797-5595
Website: www.mainebiotechnology.com

Cocalico Biologicals, Inc.
PO Box 265
449 Stevens Road
Reamstown, PA 17567-0265
Tel: 717-336-1990
Fax: 717-336-1993
Website: www.cocalicobiologicals.com

United Kingdom

Charles River Laboratories International, Inc.
Tel: +44 (0) 1843 823575
Website: www.criver.com

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

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