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AbID: 45073, Anti-ESAT6 antibody Rating: Poor Image: Anti-ESAT6 antibody for Western blot (Mycobacterium tuberculosis) Sample: Species: Mycobacterium tuberculosis Type: Purified protein Loading amount: 15 µg Specification: M.tuberculosis culture filtrate Application: Application: Gel Running Conditions: Reduced Denaturing (15% SDS-PAGE) Blocking step: Milk as blocking agent for 1 hour Blocking Concentration: 5% Other detail: Dilution: 1/4000 Incubation time: 16 hours at 4°C Diluent: 0.05% TBS-T Secondary Antibody: Name: Abcam antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Conjugation: Dilution: 1/10000 Detection: Detection method: ECL Western Blotting Substrate Exposure: 1 minute and 0 seconds Bands: Specific: ?? kDa Non-specific: above 23 kDa Additional Data:
Asked on Jul 03 2012
Thank you for submitting your recent review of ab45073. I am sorry to see that the results with this antibody were so poor.
We do fully guarantee this antibody to work in Western blot with M tuberculosis samples, so if you would like, I can send you a different antibody as a replacement for this product. I can alternatively arrange a credit or refund if you would prefer. If you'd like to accept one of these options, please let me know your original order information and I will set this up for you.
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on Jul 03 2012