Question (53042) | Anti-ESAT6 antibody (ab45073)

Go to datasheet (ab45073)

Question

AbID: 45073, Anti-ESAT6 antibody Rating: Poor Image: Anti-ESAT6 antibody for Western blot (Mycobacterium tuberculosis) Sample: Species: Mycobacterium tuberculosis Type: Purified protein Loading amount: 15 µg Specification: M.tuberculosis culture filtrate Application: Application: Gel Running Conditions: Reduced Denaturing (15% SDS-PAGE) Blocking step: Milk as blocking agent for 1 hour Blocking Concentration: 5% Other detail: Dilution: 1/4000 Incubation time: 16 hours at 4°C Diluent: 0.05% TBS-T Secondary Antibody: Name: Abcam antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Conjugation: Dilution: 1/10000 Detection: Detection method: ECL Western Blotting Substrate Exposure: 1 minute and 0 seconds Bands: Specific: ?? kDa Non-specific: above 23 kDa Additional Data:

Answer

Thank you for submitting your recent review of ab45073. I am sorry to see that the results with this antibody were so poor.

We do fully guarantee this antibody to work in Western blot with M tuberculosis samples, so if you would like, I can send you a different antibody as a replacement for this product. I can alternatively arrange a credit or refund if you would prefer. If you'd like to accept one of these options, please let me know your original order information and I will set this up for you.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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