Western blot abreview for Anti-ESAT6 antibody

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Abreviews
Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
15 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/4000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% TBS-T

Secondary antibody

Dilution
1/10000

Detection

Detection method
Pierce ECL Western Blotting Substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ?? kDa Non-specific: above 23 kDa

Additional data

Abcam response
Thank you for your valuable feedback. We are sorry to see that the antibody did not work as expected in this case. The reviewer has received a credit for their original purchase.

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Submitted Jul 02 2012

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