Mycobacterium tuberculosis Recombinant protein (Cell lysate, supernatant)
Cell lysate, supernatant
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 5% Milk in PBST
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule) antibody produced Host species: Goat Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
Specific: none kDa Non-specific: 36, 50, 64 kDa
1 hour(s), 30 minute(s) and 0 second(s)
Abcam ESAT6 full length protein (ab124574)
I only see a non-specific band between 36-64 kDa when using this antibody. My positive control, Abcam's ESAT6 full length protein, was not observed.
Thank you for submitting this Abreview. We are very sorry to hear that this antibody didn't work as expected and we are looking into this further.
We have followed up with this customer in accordance to our Abpromise.
Abcam user community
Submitted Feb 18 2014
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