Western blot abreview for Anti-ESAT6 antibody

Poor
Abreviews
Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mycobacterium tuberculosis Recombinant protein (Cell lysate, supernatant)
Specification
Cell lysate, supernatant
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 5% Milk in PBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule) antibody produced
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
chemiluminescence
Bands
Specific: none kDa Non-specific: 36, 50, 64 kDa
Exposure
1 hour(s), 30 minute(s) and 0 second(s)
Positive control
Abcam ESAT6 full length protein (ab124574)

Additional data

Additional Notes
I only see a non-specific band between 36-64 kDa when using this antibody. My positive control, Abcam's ESAT6 full length protein, was not observed.
Abcam response
Thank you for submitting this Abreview. We are very sorry to hear that this antibody didn't work as expected and we are looking into this further. We have followed up with this customer in accordance to our Abpromise. www.abcam.com/abpromise

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Verified customer

Submitted Feb 18 2014

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