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  1. Link

    esd-antibody-epr8447-ab133631.pdf

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Signal Transduction Metabolism Alcohol metabolism
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ESD antibody [EPR8447] (ab133631)

  • Datasheet
  • SDS
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Western blot - Anti-ESD antibody [EPR8447] (ab133631)
  • Western blot - Anti-ESD antibody [EPR8447] (ab133631)
  • Anti-ESD antibody [EPR8447] (ab133631)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8447] to ESD
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human ESD knockout HEK-293T cell lysate (ab257942)
Knockout
Product image
Human ESD knockout HEK-293T cell line (ab266360)

View more associated products

Overview

  • Product name

    Anti-ESD antibody [EPR8447]
    See all ESD primary antibodies
  • Description

    Rabbit monoclonal [EPR8447] to ESD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ESD aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, K562, Jurkat, Caco 2 and HeLa cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8447
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism

Associated products

  • Alternative Versions

    • Anti-ESD antibody [EPR8447] - BSA and Azide free (ab248581)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ESD knockout HEK-293T cell line (ab266360)
  • KO cell lysates

    • Human ESD knockout HEK-293T cell lysate (ab257942)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human ESD protein (ab87688)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Serine hydrolase involved in the detoxification of formaldehyde.
  • Sequence similarities

    Belongs to the esterase D family.
  • Cellular localization

    Cytoplasm. Cytoplasmic vesicle.
  • Target information above from: UniProt accession P10768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2098 Human
    • Entrez Gene: 13885 Mouse
    • Entrez Gene: 290401 Rat
    • Omim: 133280 Human
    • SwissProt: P10768 Human
    • SwissProt: Q9R0P3 Mouse
    • SwissProt: B0BNE5 Rat
    • Unigene: 432491 Human
    • Unigene: 38055 Mouse
    • Unigene: 3682 Rat
    see all
  • Alternative names

    • EC 3.1.2.12 antibody
    • Es-10 antibody
    • Es10 antibody
    • ESD antibody
    • ESTD_HUMAN antibody
    • Esterase 10 antibody
    • Esterase D antibody
    • Esterase D formylglutathione hydrolase antibody
    • FGH antibody
    • FGH, included antibody
    • FLJ11763 antibody
    • Methylumbelliferyl acetate deacetylase antibody
    • MGC139609 antibody
    • OTTHUMP00000018374 antibody
    • OTTHUMP00000040929 antibody
    • S formylglutathione hydrolase antibody
    • S-formylglutathione hydrolase antibody
    • S-formylglutathione hydrolase, included antibody
    • Sid 478 antibody
    • sid478 antibody
    see all

Images

  • Western blot - Anti-ESD antibody [EPR8447] (ab133631)
    Western blot - Anti-ESD antibody [EPR8447] (ab133631)
    All lanes : Anti-ESD antibody [EPR8447] (ab133631) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : ESD knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab133631 observed at 31 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab133631 Anti-ESD antibody [EPR8447] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266360 (knockout cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. ab133631 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ESD antibody [EPR8447] (ab133631)
    Western blot - Anti-ESD antibody [EPR8447] (ab133631)
    All lanes : Anti-ESD antibody [EPR8447] (ab133631) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Caco 2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Anti-ESD antibody [EPR8447] (ab133631)
    Anti-ESD antibody [EPR8447] (ab133631)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab133631? Please let us know so that we can cite the reference in this datasheet.

ab133631 has been referenced in 1 publication.

  • Tang M  et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233

Customer reviews and Q&As

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