Recombinant Anti-ESD antibody [EPR8447] (ab133631)
![Western blot - Anti-ESD antibody [EPR8447] (ab133631)](https://www.abcam.com/ps/products/133/ab133631/Images/ab133631-412767-anti-esd-antibody-epr8447-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ESD antibody [EPR8447] (ab133631)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8447] to ESD
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ESD antibody [EPR8447]
See all ESD primary antibodies -
Description
Rabbit monoclonal [EPR8447] to ESD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human ESD aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK293T, K562, Jurkat, Caco 2 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8447 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
Serine hydrolase involved in the detoxification of formaldehyde. -
Sequence similarities
Belongs to the esterase D family. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. - Information by UniProt
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Database links
- Entrez Gene: 2098 Human
- Entrez Gene: 13885 Mouse
- Entrez Gene: 290401 Rat
- Omim: 133280 Human
- SwissProt: P10768 Human
- SwissProt: Q9R0P3 Mouse
- SwissProt: B0BNE5 Rat
- Unigene: 432491 Human
see all -
Alternative names
- EC 3.1.2.12 antibody
- Es-10 antibody
- Es10 antibody
see all
Images
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Western blot - Anti-ESD antibody [EPR8447] (ab133631)All lanes : Anti-ESD antibody [EPR8447] (ab133631) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ESD knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaLanes 1-4: Merged signal (red and green). Green - ab133631 observed at 31 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133631 Anti-ESD antibody [EPR8447] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266360 (knockout cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. ab133631 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ESD antibody [EPR8447] (ab133631)](https://www.abcam.com/ps/products/133/ab133631/Images/ESD-Primary-antibodies-ab133631-2.jpg)
Western blot - Anti-ESD antibody [EPR8447] (ab133631)All lanes : Anti-ESD antibody [EPR8447] (ab133631) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
![Anti-ESD antibody [EPR8447] (ab133631)](https://www.abcam.com/ps/products/133/ab133631/Images/ab133631-3-benefits-of-recombinant-antibodies.png)
Anti-ESD antibody [EPR8447] (ab133631)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133631 has been referenced in 1 publication.
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233