Overview

  • Product name
  • Description
    Rabbit polyclonal to ESE1
  • Host species
    Rabbit
  • Specificity
    This antibody recognizes human ESE-1 (39-41 kD).
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    N-terminal sequence MAATCEISNIFSNYFS and C-terminal sequence SSGWKEEEVLQSRN of human ESE-1 protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ELISA 1/500 - 1/1100.
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.

Target

  • Function
    Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.
  • Tissue specificity
    Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Domain
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • E74 like factor 3 antibody
    • E74 like factor 3 ets domain transcription factor antibody
    • E74 like factor 3 ets domain transcription factor epithelial specific antibody
    • E74 like factor 3 ETS domain transcription factor serine box epithelial specific antibody
    • E74-like factor 3 antibody
    • Elf3 antibody
    • ELF3_HUMAN antibody
    • Epithelial restricted with serine box antibody
    • Epithelial-restricted with serine box antibody
    • Epithelium restricted Ets protein ESX antibody
    • Epithelium specific Ets factor 1 antibody
    • Epithelium specific Ets transcription factor 1 antibody
    • Epithelium-restricted Ets protein ESX antibody
    • Epithelium-specific Ets transcription factor 1 antibody
    • EPR 1 antibody
    • EPR1 antibody
    • ERT antibody
    • ESE-1 antibody
    • ESX antibody
    • Ets domain transcription factor serine box antibody
    • Ets domain transcription factor serine box epithelial specific antibody
    • Ets transcription factor antibody
    • ETS-related transcription factor Elf-3 antibody
    • jen antibody
    • MGC139318 antibody
    see all

References

This product has been referenced in:
  • You S  et al. An Aza resveratrol-chalcone derivative 6b protects mice against diabetic cardiomyopathy by alleviating inflammation and oxidative stress. J Cell Mol Med 22:1931-1943 (2018). Read more (PubMed: 29327811) »
  • Gu XJ  et al. Involvement of NADPH oxidases in alkali burn-induced corneal injury. Int J Mol Med 38:75-82 (2016). WB, IF . Read more (PubMed: 27221536) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply. We are sorry to hear that you are still having difficulty with this antibody. Unfortunately, we do not have another lot of this antibody to offer. We sell a fair amount of this product, so we checked with a few customers who purchased this antibody, and they were quite pleased with it. Therefore, we cannot explain why it isn't working well for you. Most likely the problems are related to the sample (Caco-2 nuclear extract), but we haven't tested this cell line, so we don't have any data to use for comparison. We are very sorry that we are unable to solve this problem. However, if you would like a refund or credit for this purchase, we'll be happy to give you that. Please do let us know how you would prefer to proceed. We look forward to hearing from you soon.

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Question

BATCH NUMBER 89608 DESCRIPTION OF THE PROBLEM Multiple non-specific bands. The anticipated size band ~40kDa is one of the weakest bands on the blot. Despite 7-fold induction in mRNA level by real-time PCR, there is no visible change in ~40kDA (presumable Ese1) band densities (inthree biological replicates) SAMPLE Human colonic carcinoma cells Caco-2, nuclear extract PRIMARY ANTIBODY Ese1 ab (Abcam ab1392) rabbit. dilution 1:1000, overnigh invubation at 4oC, washed four times 15+10+5+5 minutes SECONDARY ANTIBODY Pierce, goat anti rabbit HRP cojugated IgG at 1:20,000 dilution. 1hr at room temperature, wahed four times 15+10+5+5 minutes No primary control was not performed since this is a validated secondary antibody used in my lab many times before DETECTION METHOD Pierce SuperSignal Femto POSITIVE AND NEGATIVE CONTROLS USED Hela cells nuclear extract ANTIBODY STORAGE CONDITIONS Stored at -20oC (aliquoted upon receiving) SAMPLE PREPARATION Sample preperad in the presence of protein inhibitor cocktail (HALT, Pierce) Protein intact as tested with alternate antibodies (Sp1, Sp3) by western blot as well as EMSA. Samples mixed 1:1 with Laemli sample buffer with bME, boiled 5 min and chilled on ice prior to loading AMOUNT OF PROTEIN LOADED 30 ug nuclear protein per lane ELECTROPHORESIS/GEL CONDITIONS Reducing 10% SDS gel (Criterion, Bio-Rad) TRANSFER AND BLOCKING CONDITIONS Transferred in cold standard Tris-Glycine buffer with 20% methanol to 0.2um nitrocellulose membrane at 100V constant, 30 min. Blocked with 5% non-fat dry milk in PBS-Tween (0.05%) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? amount of leaded nuclear protein ADDITIONAL NOTES Expression and induction of Ese1 was confirmed in these cells by real-time PCR

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Answer

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. Although we have not evaluated this antibody on Caco-2 cell extracts, we have used T84 colon carcinoma cells (whole cell extracts, not nuclear extracts) and have not had the problems that you described. In our experience, multiple non-specific bands in Western blots are often attributable to one of two factors: (1) the amount of protein loaded on the gel is too high, or (2) the secondary antibody reacts non-specifically with the protein. As we see from your e-mail, you loaded 30 ug of protein on his gel. For testing this particular antibody, we loaded only 5 ug of protein and diluted the ESE-1 antibody 1:500. We see also that you did not test the secondary antibody, without primary antibody, on the blots because you have confidence in your secondary antibody. This control really should be included in order to eliminate the possibility that the secondary binds non-specifically or has unexpected cross-reactivity with the sample. Perhaps you will agree to try the assay again with a lower amount of sample and the "no primary" control? We hope this will help. Should you still have any problem, then please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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