Anti-ESE1 antibody (ab194943)
Key features and details
- Rabbit polyclonal to ESE1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ESE1 antibody
See all ESE1 primary antibodies -
Description
Rabbit polyclonal to ESE1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Gorilla, Orangutan
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Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human ESE1 aa 1-100 (N terminal). NCBI Accession No. NP_004424.3.
Database link: P78545
Synthetic peptide corresponding to Human ESE1 aa 350 to the C-terminus (C terminal). NCBI Accession No. NP_004424.3.
Database link: P78545 -
Positive control
- Recombinant Human ESE1 protein (ab131849) can be used as a positive control in WB. Human breast tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. -
Tissue specificity
Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Cellular localization
Cytoplasm. Nucleus. Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism. - Information by UniProt
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Database links
- Entrez Gene: 421177 Chicken
- Entrez Gene: 508503 Cow
- Entrez Gene: 490235 Dog
- Entrez Gene: 101127911 Gorilla
- Entrez Gene: 100146645 Horse
- Entrez Gene: 1999 Human
- Entrez Gene: 13710 Mouse
- Entrez Gene: 100174422 Orangutan
see all -
Alternative names
- E74 like factor 3 antibody
- E74 like factor 3 ets domain transcription factor antibody
- E74 like factor 3 ets domain transcription factor epithelial specific antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ESE1 antibody (ab194943)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue, labeling ESE1 using ab194943 at 5 µg/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab194943 has not yet been referenced specifically in any publications.