Product nameAnti-ESE1 antibody [ELF3B10C4]
See all ESE1 primary antibodies
DescriptionMouse monoclonal [ELF3B10C4] to ESE1
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
Recombinant fragment (Human)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesProtein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22µm membrane
Our Abpromise guarantee covers the use of ab50746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 41 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionTranscriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.
Tissue specificityExpressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Cellular localizationCytoplasm. Nucleus. Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism.
- Information by UniProt
- E74 like factor 3 antibody
- E74 like factor 3 ets domain transcription factor antibody
- E74 like factor 3 ets domain transcription factor epithelial specific antibody
Lane 1 : Molecular weight markers
Lane 2 : Anti-ESE1 antibody [ELF3B10C4] (ab50746)
Lane 2 : Immunising peptide
Predicted band size: 41 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ab50746 has not yet been referenced specifically in any publications.