Overview

  • Product name

  • Description

    Rabbit polyclonal to ESM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-184 of Human ESM1 (NP_008967.1).

  • Positive control

    • Human kidney tissue lysate; ESM1 transfected 293T cell line lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    May have potent implications in lung endothelial cell-leukocyte interactions.
  • Tissue specificity

    Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
  • Post-translational
    modifications

    May contain intrachain disulfide bonds.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endocan antibody
    • Endothelial cell specific molecule 1 antibody
    • Endothelial cell-specific molecule 1 antibody
    • ESM 1 antibody
    • ESM 1 secretory protein antibody
    • ESM-1 antibody
    • Esm1 antibody
    • ESM1 secretory protein antibody
    • ESM1_HUMAN antibody
    see all

Images

  • Anti-ESM1 antibody (ab103590) at 1/500 dilution + Human kidney tissue lysate at 50 µg

    Predicted band size: 20 kDa

  • All lanes : Anti-ESM1 antibody (ab103590) at 1/500 dilution

    Lane 1 : ESM1 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 20 kDa

  • ab103590 staining ESM1 in the Human HUVEC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit monoclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Vilà-González M  et al. Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling. Stem Cells 37:226-239 (2019). Read more (PubMed: 30372556) »
  • Lee YH  et al. Endocan as a marker of microvascular inflammation in kidney transplant recipients. Sci Rep 9:1854 (2019). Read more (PubMed: 30755622) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Brain
Blocking step
Caesin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 19 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 26 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Specification
HUVEC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 28 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up