Esomeprazole sodium, H+/ K+-ATPase (proton pump) inhibitor (ab120500)
Overview
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Product name
Esomeprazole sodium, H+/ K+-ATPase (proton pump) inhibitor -
Description
H+/ K+-ATPase (proton pump) inhibitor -
Biological description
H+ / K+-ATPase (proton pump) inhibitor (IC50 = 2.3 µM). S-enantiomer of omeprazole. Attenuates intestinal mucosal barrier damage and can promote healing of gastric lesions in rats. Also inhibits Cytochrome P450 2C19 (CYP2C9), CYP2C19 and CYP3A4 (Ki values are 81.5, 8.6 and 46.6 µM, respectively). -
Purity
> 99% -
CAS Number
161796-78-7 -
Chemical structure
Properties
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Chemical name
6-Methoxy-2-[(S)-[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-1H-benzimidazole sodium salt -
Molecular weight
367.40 -
Molecular formula
C17H18N3NaO3S -
PubChem identifier
23674541 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM, in ethanol to 100 mM and in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Na+].Cc3c(OC)c(C)cnc3CS(=O)c1[n-]c2cc(ccc2n1)OC -
Source
Synthetic
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Research areas
Applications
Our Abpromise guarantee covers the use of ab120500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - Esomeprazole sodium, H+/ K+-ATPase (proton pump) inhibitor (ab120500)MDA-MB-231 cells were incubated at 37°C for 6h with vehicle control (0 µM) and 100 µM of esomeprazole sodium (ab120500). Increased expression of aryl hydrocarbon receptor (ab2770) correlates with an increase in esomeprazole sodium concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab2770 at 2 µg/ml and ab8226 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab120500 has not yet been referenced specifically in any publications.
