Anti-Estradiol antibody [AB4.2] (ab116648)

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Overview

  • Product name

    Anti-Estradiol antibody [AB4.2]
    See all Estradiol primary antibodies

  • Description

    Mouse monoclonal [AB4.2] to Estradiol

  • Host species

    Mouse

  • Specificity

    ab116648 shows reactivity with Chemical samples.

  • Tested applications

    Suitable for: IP, ELISA, RIAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Estradiol-6-CMO-KLH

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.019% Potassium chloride, 0.79% Sodium chloride, 0.135% Potassium phosphate, 0.1% BSA
  • Concentration information loading...

  • Purity

    DEAE-Chromatography

  • Clonality

    Monoclonal

  • Clone number

    AB4.2

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.

Target

  • Relevance

    Estradiol is the most potent estrogen of a group of endogenous estrogen steroids which includes estrone and estriol. In women estradiol is responsible for growth of the breast and reproductive epithelia, maturation of long bones and development of the secondary sexual characteristics. Estradiol is produced mainly by the ovaries with secondary production by the adrenal glands and conversion of steroid precursors into estrogens in fat tissue.

  • Cellular localization

    Secreted

  • Alternative names

    • 17ß estradiol antibody
    • E2 antibody
    • Ethynyl Estradiol antibody
    • Oestradiol antibody

References

ab116648 has not yet been referenced specifically in any publications.

Publishing research using ab116648? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

, I'm trying to measure human estradiol in salivary samples using 3H RIA.  I ordered an antibody from MP Biomedicals, only to discover that it was too dilute for my assay.  Is there any chance I could purchase a small volume of this antibody to make sure it will run in my system? Thanks in advance!

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Answer

Thank you for contacting Abcam. We do not stock any sample sizes of our antibodies, but we do cover our antibodies under our Abpromise. In this case ab116648, anti-Estradiol antibody, is guaranteed to work in RIA applications. ab116648 has a total of 100ug at a concentration of 1mg/ml, hopefully this would be at a high enough concentration for your needs. If there is anything else I can help you with, please let me know.

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