Recombinant Anti-Estriol antibody [EPPTXR3] (ab150407)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTXR3] to Estriol
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Estriol antibody [EPPTXR3]
See all Estriol primary antibodies -
Description
Rabbit monoclonal [EPPTXR3] to Estriol -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTXR3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Competitive ELISA |
Use a concentration of 0.075 µg/ml.
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Notes |
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Competitive ELISA
Use a concentration of 0.075 µg/ml. |
Target
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Relevance
Estriol is a hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3-beta, 16-alpha, and 17-beta position. Estriol is the main estrogen in the blood and urine of pregnant females. During pregnancy, large amount of estriol is produced by the placenta. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function. Estriol increases until term, reaches a plateau at the 40th week of gestation and then declines. Isomers with inversion of the hydroxyl group or groups are called epiestriol. It is used in the treatment of osteoporosis in combination with Vitamin D. Its expression also seems to be one of the direct causes of preterm labour, together with cervical ultrasound mesurements and espression of Foetal Fibronectin. -
Cellular localization
Secreted -
Alternative names
- E3 antibody
Images
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2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1%BSA, serial dilution of Estriol (0, 0.1, 0.5, 2, 5, 10, 20, and 50ng/ml) were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96-wells. After washing, TMB substrate was used to develop the color.
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2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1% BSA, 20ng/ml of various buffer containing Estriol, Estradiol, Estrone, 16-Epiestriol, Estriol 3-sulfate, 16-Hydroxyestrone , or PBS buffer only were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96- wells. After washing, TMB substrate was used to develop the color.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150407 has not yet been referenced specifically in any publications.