Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTXR3] to Estriol
- Suitable for: ELISA
- Reacts with: Species independent
Product nameAnti-Estriol antibody [EPPTXR3]
See all Estriol primary antibodies
Species reactivityReacts with: Species independent
Other Immunogen Type corresponding to Estriol conjugated to keyhole limpet haemocyanin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
|ELISA||Use a concentration of 0.075 µg/ml.|
RelevanceEstriol is a hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3-beta, 16-alpha, and 17-beta position. Estriol is the main estrogen in the blood and urine of pregnant females. During pregnancy, large amount of estriol is produced by the placenta. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function. Estriol increases until term, reaches a plateau at the 40th week of gestation and then declines. Isomers with inversion of the hydroxyl group or groups are called epiestriol. It is used in the treatment of osteoporosis in combination with Vitamin D. Its expression also seems to be one of the direct causes of preterm labour, together with cervical ultrasound mesurements and espression of Foetal Fibronectin.
- E3 antibody
2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1%BSA, serial dilution of Estriol (0, 0.1, 0.5, 2, 5, 10, 20, and 50ng/ml) were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96-wells. After washing, TMB substrate was used to develop the color.
2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1% BSA, 20ng/ml of various buffer containing Estriol, Estradiol, Estrone, 16-Epiestriol, Estriol 3-sulfate, 16-Hydroxyestrone , or PBS buffer only were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96- wells. After washing, TMB substrate was used to develop the color.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab150407 has not yet been referenced specifically in any publications.