Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTXR3] to Estriol - Low endotoxin, Azide free
- Suitable for: ELISA
- Reacts with: Species independent
Product nameAnti-Estriol antibody [EPPTXR3] - Low endotoxin, Azide free
See all Estriol primary antibodies
DescriptionRabbit monoclonal [EPPTXR3] to Estriol - Low endotoxin, Azide free
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Species independent
Other Immunogen Type conjugated to keyhole limpet haemocyanin.
ab221960 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab221960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
RelevanceEstriol is a hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3-beta, 16-alpha, and 17-beta position. Estriol is the main estrogen in the blood and urine of pregnant females. During pregnancy, large amount of estriol is produced by the placenta. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function. Estriol increases until term, reaches a plateau at the 40th week of gestation and then declines. Isomers with inversion of the hydroxyl group or groups are called epiestriol. It is used in the treatment of osteoporosis in combination with Vitamin D. Its expression also seems to be one of the direct causes of preterm labour, together with cervical ultrasound mesurements and espression of Foetal Fibronectin.
- E3 antibody
This ELISA data was generated with the same anti-Estriol antibody clone [EPPTXR3] in a different buffer formulation (cat# ab150407).
2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1%BSA, serial dilution of Estriol (0, 0.1, 0.5, 2, 5, 10, 20, and 50ng/ml) were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96-wells. After washing, TMB substrate was used to develop the color.
This ELISA data was generated using the same anti-Estriol antibody clone [EPPTXR3] in a different buffer formulation (cat# ab150407).
2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1% BSA, 20ng/ml of various buffer containing Estriol, Estradiol, Estrone, 16-Epiestriol, Estriol 3-sulfate, 16-Hydroxyestrone , or PBS buffer only were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96- wells. After washing, TMB substrate was used to develop the color.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab221960 has not yet been referenced specifically in any publications.