Overview

  • Product name

    Estriol ELISA Kit 
    See all Estriol kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Saliva 16 <=9.7%
    Inter-assay
    Sample n Mean SD CV%
    Saliva 10 <=13.7%
  • Sample type

    Saliva
  • Assay type

    Competitive
  • Sensitivity

    1 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 104 50pg/ml - 300pg/ml

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in saliva.
    A 96-well plate has been precoated with anti-Estriol antibodies (solid-phase). Samples and the Estriol HRP Conjugate are added to the wells, where Estriol in the sample competes with the added Estriol HRP Conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Protocols

References

ab178662 has not yet been referenced specifically in any publications.

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