Anti-Estrogen Receptor alpha antibody (ab3575)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Estrogen Receptor alpha antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment
Predicted to work with: Hamster, Cow, Cat -
Immunogen
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Positive control
- WB: recombinant fragment; IHC: rat prostate tissue; ICC/IF: T47D and 578T breast cancer cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 1 - 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).
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ICC/IF |
1/1000 - 1/4000.
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Notes |
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IHC-P
Use a concentration of 1 - 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847). |
ICC/IF
1/1000 - 1/4000. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 407238 Cow
- Entrez Gene: 101825539 Hamster
- Entrez Gene: 2099 Human
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P49884 Cow
- SwissProt: Q9QZJ5 Hamster
- SwissProt: P03372 Human
see all -
Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
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All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDaBlocked with 5% milk in TBST for at least 1 hour at room temperature.
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4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab3575 has been referenced in 33 publications.
- Salvati A et al. Combinatorial targeting of a chromatin complex comprising Dot1L, menin and the tyrosine kinase BAZ1B reveals a new therapeutic vulnerability of endocrine therapy-resistant breast cancer. Breast Cancer Res 24:52 (2022). PubMed: 35850772
- Song T et al. Zearalenone Promotes Uterine Development of Weaned Gilts by Interfering with Serum Hormones and Up-Regulating Expression of Estrogen and Progesterone Receptors. Toxins (Basel) 14:N/A (2022). PubMed: 36355982
- Kamal DAM et al. Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model. Int J Mol Sci 23:N/A (2022). PubMed: 36499085
- Broome R et al. TET2 is a component of the estrogen receptor complex and controls 5mC to 5hmC conversion at estrogen receptor cis-regulatory regions. Cell Rep 34:108776 (2021). PubMed: 33626359
- Liu J et al. Maternal Diabetes-Induced Suppression of Oxytocin Receptor Contributes to Social Deficits in Offspring. Front Neurosci 15:634781 (2021). PubMed: 33633538