Question (26959) | Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063)

Go to datasheet (ab32063)

Question

Weak staining in MCF7 cells.

Answer

Hopefully the protocol tips that we talked about today will help improve the weak signal you are seeing using ab32063. The protocol tips that we discussed are: Fix in 4% PFA for 10 minutes in room temperature. Use 1/50 dilution of the primary antibody. Try a different secondary antibody. Please let me know if you are still having issue with the antibody after trying out the above tips. If there is anything else I can do please let me know.

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