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Thank you for your quick reply. Your suggestion for performing individual staining beforehand is appreciable. AsI will use frozen tissue of rats to perform the double fluorescence staining and not by paraffin blocked tissue. I was able to find some info for the other antibodies that were used for frozen tissue and not fot the antibodies that I requested. So, could you also, confirm me about the usability of the same antibodies for the frozen tissue.
Asked on Jul 12 2012
Thank you for your response.
Both antibodies detect rat species and recognize the native form of Estrogen Receptor alpha and beta. These antibodies have been tested on formalin-fixed paraffin-embedded tissue sections and it is very likely that they are suitable for IHC on frozen sections as well.
Generally speaking, if an antibody is applicable in IHC-P, it is very likely to work in IHC-Fr. However, if the antibody has only been tested in IHC-Fr, we can't predict the suitability for IHC-P.
I hope this will be useful for you.
Answered on Jul 12 2012