Flow Cytometry abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 647)

Below Average
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (Spleen T cells)
Permeabilization
Yes - eBioscience™ Permeabilization Buffer
Gating Strategy
CD45+ CD3+ CD4- CD8+
Specification
Spleen T cells
Preparation
Cell harvesting/tissue preparation method: Mechanical disruption
Sample buffer: PBS w/o Ca or Mg
Fixation
Paraformaldehyde

Other product details

Incubation time
45 minute(s) · Diluent: Perm buffer, 2% donkey serum
Dilution
1/20

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
Sample: Spleen cells isolated from UBC-Cre-ERT2 mouse Almost no staining with 1:50 dilution, only a little signal increase when 1:20 dilution was used Red - unstained cells Blue - 1:50 Orange - 1:20
Abcam response
ab205851 hasn't been tested in flow cytometry application with mouse sample. The end user has tested it under our Abtrial offer, a free of charge product has been offered in exchange of this review.

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Submitted Mar 02 2018

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