Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
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- Specific References
- Protocols
Overview
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Product nameAnti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free
See all Estrogen Receptor alpha primary antibodies -
DescriptionRabbit monoclonal [EPR4097] to Estrogen Receptor alpha - BSA and Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, ChIP, IHC-P, WB, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Estrogen Receptor alpha aa 1-300.
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General notes
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR4097
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade (ab108398)
- Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 488) (ab205850)
- Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 647) (ab205851)
- Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 594) (ab207046)
- Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 568) (ab207261)
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab167610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ICC/IF | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Antigen retrieval is recommended |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 66 kDa. | |
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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Alternative names
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
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Protocols
References
ab167610 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"