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  1. Link

    estrogen-receptor-alpha-antibody-sp1-ab16660.pdf

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RecombinantRabMAb

Recombinant Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

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  • SDS
Reviews (7)Q&A (2)References (50)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Western blot - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP1] to Estrogen Receptor alpha
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE

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Overview

  • Product name

    Anti-Estrogen Receptor alpha antibody [SP1]
    See all Estrogen Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [SP1] to Estrogen Receptor alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Epitope

    C-terminal
  • Positive control

    • WB: MCF7 cell lysate. IHC-P: Human breast carcinoma, cervix, breast, breast ductal carcinoma and ovarian adenocarcinoma tissue. ICC/IF: MCF7 cells. Flow Cyt (intra): MCF7 cells.
  • General notes

    This product has switched from a hybridoma to recombinant production format on 21st May 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP1
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen
    • Cancer
    • Tumor biomarkers
    • Receptors

Associated products

  • Alternative Versions

    • Anti-Estrogen Receptor alpha antibody [SP1] - BSA and Azide free (ab187260)
    • Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [SP1] (ab267512)
  • Assay kits

    • Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (ab207203)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)
  • Recombinant Protein

    • Recombinant Human Estrogen Receptor alpha protein (ab82606)
  • Related Products

    • Recombinant Human Estrogen Receptor alpha protein (ab82606)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/200.
WB
1/25. Predicted molecular weight: 67 kDa.

Incubate for 1 hour at room temperature.

IHC-P (2)
1/200.
ICC/IF (1)
1/25 - 1/250.
Notes
Flow Cyt (Intra)
1/200.
WB
1/25. Predicted molecular weight: 67 kDa.

Incubate for 1 hour at room temperature.

IHC-P
1/200.
ICC/IF
1/25 - 1/250.

Target

  • Function

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Target information above from: UniProt accession P03372 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2099 Human
    • Entrez Gene: 13982 Mouse
    • Omim: 133430 Human
    • SwissProt: P03372 Human
    • SwissProt: P19785 Mouse
    • Unigene: 208124 Human
    • Unigene: 463262 Mouse
    • Unigene: 9213 Mouse
    • Alternative names

      • 7*/654 isoform antibody
      • 7*/819 2 isoform antibody
      • 7*/822 isoform antibody
      • 8*/901 isoform antibody
      • 8*/941 isoform antibody
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      IHC image of ab16660 staining Estrogen Receptor alpha in normal human cervix formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16660, 1/250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      This image was generated using the hybridoma version of the product.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      ab16660 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab16660 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Western blot - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Western blot - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) at 1/25 dilution + lysate prepared from MCF7 cells

      Predicted band size: 67 kDa



      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Image courtesy of Joseph J D et al. eLife 2016;5:e15828 doi: 10.7554/eLife.15828

      Immunohistochemical analysis of tamoxifen resistant MCF7 xenograft tumours staining estrogen receptor alpha with ab16660. The mice used were treated with a vehicle, tamoxifen (120mg/kg/day p.o) or GDC-0810 (100mg/kg/day p.o.) for 27 days.

      This image was generated using the hybridoma version of the product.

    • Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Intracellular flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling Estrogen Receptor alpha with purified ab16660 at 1/200 dilution (1.06 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).  This image was generated using the hybridoma version of the product.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Estrogen Receptor alpha with purified ab16660 at 1/25 (8.5 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Formalin-fixed, paraffin-embedded human breast tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

      Human breast carcinoma stained with ab16660.

      This image was generated using the hybridoma version of the product.

    • Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
      Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (50)

    Publishing research using ab16660? Please let us know so that we can cite the reference in this datasheet.

    ab16660 has been referenced in 50 publications.

    • Weng Y  et al. miR-133b has protective effect on rats with acute lung injury caused by severe acute pancreatitis through targeting sp1 gene. Int J Clin Exp Pathol 14:86-96 (2021). PubMed: 33532026
    • Li W  et al. A synergetic effect of BARD1 mutations on tumorigenesis. Nat Commun 12:1243 (2021). PubMed: 33623049
    • Kapara A  et al. Detection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS. Anal Chem 93:5862-5871 (2021). PubMed: 33797884
    • Kamiya Y  et al. Puerarin blocks the aging phenotype in human dermal fibroblasts. PLoS One 16:e0249367 (2021). PubMed: 33886592
    • Zhou H  et al. Chaperone-mediated Autophagy Governs Progression of Papillary Thyroid Carcinoma via PPAR?-SDF1/CXCR4 Signaling. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32556197
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Ovary)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 and pH9
    Permeabilization
    Yes - Triton X-100
    Specification
    Ovary
    Blocking step
    Vector Protein free blocker as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 01 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: BOND ER2
    Permeabilization
    No
    Specification
    breast cancer
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Masahiro Hirata

    Verified customer

    Submitted Dec 13 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (stable Sirt-3 overexpressing MCF-7 breast cancer c)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    stable Sirt-3 overexpressing MCF-7 breast cancer c
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sandra Sobocanec

    Verified customer

    Submitted Jan 15 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Intestine)
    Permeabilization
    Yes - 0.1% Triton 0.05% Tween
    Specification
    Intestine
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Agata Szade

    Verified customer

    Submitted Mar 08 2018

    Flow Cytometry abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Spleen T cells)
    Permeabilization
    Yes - eBioscience™ Permeabilization Buffer
    Gating Strategy
    CD45+ CD3+ CD4+ CD8-
    Specification
    Spleen T cells
    Preparation
    Cell harvesting/tissue preparation method: Mechanical disruption
    Sample buffer: PBS w/o Ca or Mg
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 07 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis)
    Permeabilization
    Yes - 0.04% tween
    Specification
    Testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    俊徳 中川

    Verified customer

    Submitted Jun 13 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (a CreERT2 fusion gene (Cre-ERT2 Cre recombinase fu)
    Permeabilization
    Yes - with 0.1% triton X-100 for 10 minutes
    Specification
    a CreERT2 fusion gene (Cre-ERT2 Cre recombinase fu
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 10 2015

    Question

    What is the difference between ab27614 and ab16660?
    Do you have any IHC kits?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 03 2012

    Answer

    Thank you for your telephone enquiry this morning.


    As discussed on the telephone, I can confirm these antibodies are different and will be detecting different eptiopes. They have also been tested ina slightly different list of applications and species.One other major difference is that ab16660is sold as tissue culture supernatant, ab27614 is purified.

    During our discussions, you mentioned these may be used for diagnostics. Please notethat regrettablyour products are not accredited for clinical diagnostic use.However, we canprovide our 6 month guarantee for the applications and species listed on the individual datasheets.

    We do sell a large range of IHC reagents now which I hope will be helpful to you. They can be found by searching on our website, or you may find it helpful to click on the links from the following IHC reagentspage:

    https://www.abcam.com/index.html?pageconfig=resource&rid=12853

    I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Aug 03 2012

    Question

    Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Anti-Myeloperoxidase antibody (ab45977) 5. Anti-TdT antibody (ab85148) 6. Anti-TMEM16A antibody (ab53212) 7. Anti-NSE antibody [EPR3377] (ab79757) 8. Anti-CD23 antibody [EPR3617(IHC)] (ab92495) 9. Anti-CD10 antibody [56C6] (ab951) 10. Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) 11. Anti-CD79a antibody [HM47/A9] (ab3121) 12. Anti-CD45RO antibody [UCHL-1] (ab86080) 13. Anti-CD45 antibody (ab10559) 14. Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) 15. Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2012

    Answer

    Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html (or use the following: https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html (or use the following: https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html (or use the following: https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html (or use the following: https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html (or use the following: https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

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    Abcam Scientific Support

    Answered on Jan 09 2012

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