Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
- Datasheet
- References (19)
- Protocols
Overview
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Product nameAnti-Estrogen Receptor alpha antibody [SP1]
See all Estrogen Receptor alpha primary antibodies -
DescriptionRabbit monoclonal [SP1] to Estrogen Receptor alpha
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-FoFrmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Pig
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Immunogen
Synthetic peptide corresponding to Human Estrogen Receptor alpha (C terminal).
Database link: P03372 -
EpitopeC-terminal
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Positive control
- WB: MCF7 cell lysate. IHC-P: Human breast carcinoma, cervix, breast, breast ductal carcinoma and ovarian adenocarcinoma tissue. ICC/IF: MCF7 cells. IHC-Fr: Mouse intestine tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.50
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, Tris buffered saline -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberSP1
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab16660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/25. Predicted molecular weight: 67 kDa. Incubate for 1 hour at room temperature. |
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| IHC-P | 1/200. | |
| ICC/IF | 1/250. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 29403069 |
Target
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FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Entrez Gene: 397435 Pig
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- SwissProt: Q29040 Pig
- Unigene: 208124 Human
see all -
Alternative names
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-250458-ICCGR202593101.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)ab16660 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab16660 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-250460-IHCGR194259101.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)IHC image of ab16660 staining Estrogen Receptor alpha in normal human cervix formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16660, 1/250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/Estrogen-Receptor-Primary-antibodies-ab16660-1.jpg)
Western blot - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) at 1/25 dilution + lysate prepared from MCF7 cells
Predicted band size: 67 kDa -
![Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-318448-64.jpg)
Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)This image is courtesy of an Abreview from Dr. Agata Szade.Immunohistochemical analysis of paraformaldehyde-fixed, 0.1% Triton 0.05% Tween permeabilized frozen Mouse Tissue sections (Intestine) stained for Estrogen Receptor alpha (purple) using ab16660 at 1/100 dilution. Primary was incubated for 16 hours at 4°C with serum as blocking agent for 2 hours at 20°C.
Secondary antibody was AffiniPure F(ab')2 Fragment Alexa Fluor® 647-conjugated Donkey anti-rabbit IgG at 1/500 dilution.HPRT-Cre/ERT2 x mT/mG mouse, treated with tamoxifen.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-284730-ab16660--IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-284732-ab16660--IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-284734-ab16660--IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Formalin-fixed, paraffin-embedded human breast tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/ab16660-284736-ab16660--IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)](https://www.abcam.com/ps/products/16/ab16660/Images/abcam1-16660.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Human breast carcinoma stained with ab16660.
Protocols
References
This product has been referenced in:
- He BX et al. Association between UDP-glucuronosyltransferase 2B7 tagSNPs and breast cancer risk in Chinese females. Clin Exp Pharmacol Physiol 45:437-443 (2018). Read more (PubMed: 29272031) »
- Guo C et al. microRNA-10b expression and its correlation with molecular subtypes of early invasive ductal carcinoma. Exp Ther Med 15:2851-2859 (2018). Read more (PubMed: 29599829) »
