Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (a CreERT2 fusion gene (Cre-ERT2 Cre recombinase fu)
Permeabilization
Yes - with 0.1% triton X-100 for 10 minutes
Specification
a CreERT2 fusion gene (Cre-ERT2 Cre recombinase fu
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal donkey serum in 1x PBS pH 7.4
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa-fluorescent 488 donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
ABTRIAL-TVB97 The antibody was used to identify CreERT2 expressing cells that had not been induced. The tissue was kidney from a WT1CreERT2 mouse which expresses a CreERT2 fusion gene (Cre-ERT2 Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain) from the WT1 (Wilm's tumor antigen 1) locus.

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Submitted Aug 10 2015

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