Flow Cytometry abreview for Anti-Estrogen Receptor alpha antibody [SP1]

Average
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (Spleen T cells)
Permeabilization
Yes - eBioscience™ Permeabilization Buffer
Gating Strategy
CD45+ CD3+ CD4+ CD8-
Specification
Spleen T cells
Preparation
Cell harvesting/tissue preparation method: Mechanical disruption
Sample buffer: PBS w/o Ca or Mg
Fixation
Paraformaldehyde

Other product details

Incubation time
45 minute(s) · Diluent: Perm buffer, 2% donkey serum
Dilution
1/20

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: AffiniPure F(ab')2 Fragment Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

Additional data

Additional Notes
Sample: Spleen cells isolated from UBC-Cre-ERT2 mouse There is some staining in the ER+ sample, but also (to much lesser extent) in the ER- cells not a very good separation between the negative and a positive sample Orange - secondary antibody only (negative control) Red - UBC-Cre-ERT2- Blue - UBC-Cre-ERT2+

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Verified customer

Submitted Mar 07 2018

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