Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody [SP1]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Intestine)
Permeabilization
Yes - 0.1% Triton 0.05% Tween
Specification
Intestine
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% blocking buffer
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: AffiniPure F(ab')2 Fragment Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

Additional data

Additional Notes
HPRT-Cre/ERT2 x mT/mG mouse, treated with tamoxifen

Dr. Agata Szade

Verified customer

Submitted Mar 08 2018

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