Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Receptor alpha antibody [SP1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (stable Sirt-3 overexpressing MCF-7 breast cancer c)
Permeabilization
Yes - 0.1% Triton X
Specification
stable Sirt-3 overexpressing MCF-7 breast cancer c
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: 1% BSA + 0.1% Triton X + PBS (pH=7.4)
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat-Anti Rabbit IgG (H+L) Cross-Adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Dr. Sandra Sobocanec

Verified customer

Submitted Jan 15 2019

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