Product nameAnti-Estrogen Receptor alpha antibody [SP1], prediluted
See all Estrogen Receptor alpha primary antibodies
DescriptionRabbit monoclonal [SP1] to Estrogen Receptor alpha, prediluted
Specificityab27595 recognises estrogen receptor alpha (ER).
Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig
Synthetic peptide within Human Estrogen Receptor alpha aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P03372
- Breast carcinomas
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab27595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 29403069|
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
USE NEAT. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
Immunohistochemical staining of human breast carcinoma labelling Estrogen Receptor alpha [SP1] with ab93739 at 1:200 dilution.
Staining of human breast carcinoma using ab27595
Anti-Estrogen Receptor alpha antibody [SP1], prediluted (ab27595) at 1/25 dilution + lysate prepared from MCF7 cells
Image produced using the diluted version of this antibody, ab16660.
This product has been referenced in:
- Li Y et al. Genetic targeting of Purkinje fibres by Sema3a-CreERT2. Sci Rep 8:2382 (2018). IHC-FoFr ; Mouse . Read more (PubMed: 29403069) »
- Gu J et al. Knockdown of HIF-1a by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer. Sci Rep 7:7546 (2017). Read more (PubMed: 28790395) »