Key features and details
- Mouse monoclonal [TE111.5D11] to Estrogen Receptor alpha
- Suitable for: ChIP, WB, IP, GSA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Estrogen Receptor alpha antibody [TE111.5D11]
See all Estrogen Receptor alpha primary antibodies
DescriptionMouse monoclonal [TE111.5D11] to Estrogen Receptor alpha
Tested applicationsSuitable for: ChIP, WB, IP, GSAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Pig
Recombinant fragment corresponding to Human Estrogen Receptor alpha. Recombinant fragment, corresponding to amino acids 302-595 of Human Estrogen Receptor alpha.
Database link: P11474
- WB and Flow Cyt: MCF-7 cells.
The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab16460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).|
|IP||Use at 2 µg/mg of lysate.|
|GSA||Use at an assay dependent concentration.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
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ab16460 has been referenced in 7 publications.
- Wang B et al. A dual role of miR-22 modulated by RelA/p65 in resensitizing fulvestrant-resistant breast cancer cells to fulvestrant by targeting FOXP1 and HDAC4 and constitutive acetylation of p53 at Lys382. Oncogenesis 7:54 (2018). PubMed: 30057418
- Cho KA et al. Mesenchymal stem cells ameliorate B-cell-mediated immune responses and increase IL-10-expressing regulatory B cells in an EBI3-dependent manner. Cell Mol Immunol N/A:N/A (2017). PubMed: 28042143
- Deng X et al. Guanxin Danshen Formulation Protects against Myocardial Ischemia Reperfusion Injury-Induced Left Ventricular Remodeling by Upregulating Estrogen Receptor ß. Front Pharmacol 8:777 (2017). PubMed: 29163163
- Elbaradie KB et al. Sex-specific response of rat costochondral cartilage growth plate chondrocytes to 17ß-estradiol involves differential regulation of plasma membrane associated estrogen receptors. Biochim Biophys Acta 1833:1165-72 (2013). PubMed: 23305904
- Marmugi A et al. Low doses of bisphenol A induce gene expression related to lipid synthesis and trigger triglyceride accumulation in adult mouse liver. Hepatology 55:395-407 (2012). PubMed: 21932408
- Pedram A et al. Developmental phenotype of a membrane only estrogen receptor alpha (MOER) mouse. J Biol Chem 284:3488-95 (2009). PubMed: 19054762
- Safdar A et al. miRNA in the regulation of skeletal muscle adaptation to acute endurance exercise in C57Bl/6J male mice. PLoS One 4:e5610 (2009). WB ; Human . PubMed: 19440340