Product nameAnti-Estrogen Receptor alpha antibody [TE111.5D11]
See all Estrogen Receptor alpha primary antibodies
DescriptionMouse monoclonal [TE111.5D11] to Estrogen Receptor alpha
Tested applicationsSuitable for: Flow Cyt, IP, WB, Neutralisingmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Estrogen Receptor alpha aa 302-595. Recombinant fragment, corresponding to amino acids 302-595 of Human Estrogen Receptor alpha.
Database link: P03372
- MCF-7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab16460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration. Use at a concentration of 2 µg/mg of protein lysate on native and denatured lysate.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).|
|Neutralising||Use at an assay dependent concentration. This antibody can be used to block binding of estrogen to the estrogen receptor.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
ab16460 at 1
g/ml detecting Estrogen Recptor from MCF-7 cells by Western blot. ab16460 at 1µg/ml detecting Estrogen Recptor from MCF-7 cells by Western blot. µ
Overlay histogram showing MCF7 cells stained with ab16460 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16460, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Deng X et al. Guanxin Danshen Formulation Protects against Myocardial Ischemia Reperfusion Injury-Induced Left Ventricular Remodeling by Upregulating Estrogen Receptor ß. Front Pharmacol 8:777 (2017). Read more (PubMed: 29163163) »
- Elbaradie KB et al. Sex-specific response of rat costochondral cartilage growth plate chondrocytes to 17ß-estradiol involves differential regulation of plasma membrane associated estrogen receptors. Biochim Biophys Acta 1833:1165-72 (2013). Read more (PubMed: 23305904) »