Recombinant
RabMAb

Recombinant Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody [EPR1827] (ab75753)

Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody [EPR1827]
    See all Estrogen Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR1827] to Estrogen Receptor alpha (phospho S104 + S106)
  • Host species

    Rabbit
  • Specificity

    ab75753 only detects Estrogen Receptor alpha phosphorylated on Serine 104/106.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Serine 104/106 of human Estrogen Receptor alpha.

  • Positive control

    • MCF-7 cell lysates.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Post-translational
      modifications

      Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).
    • Cellular localization

      Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Information by UniProt
    • Database links

    • Alternative names

      • 7*/654 isoform antibody
      • 7*/819 2 isoform antibody
      • 7*/822 isoform antibody
      • 8*/901 isoform antibody
      • 8*/941 isoform antibody
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    Images

    • All lanes : Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody [EPR1827] (ab75753) at 1/2000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates. Then the membrane was incubated with lambda phosphatase.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 66 kDa
      Observed band size: 66 kDa


      Exposure time: 180 seconds


      Blocking buffer and concentration 5% NFDM/TBST
      Diluting buffer and concentration 5% NFDM/TBST

    References

    This product has been referenced in:

    • Held JM  et al. Ligand binding promotes CDK-dependent phosphorylation of ER-alpha on hinge serine 294 but inhibits ligand-independent phosphorylation of serine 305. Mol Cancer Res 10:1120-32 (2012). Read more (PubMed: 22669764) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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