Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S106)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Estrogen Receptor alpha (phospho S106) antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha (phospho S106) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF cells; MCF7 cell extracts treated with EGF; Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131111 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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IHC-P | (1) |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P06211 Rat
- Unigene: 208124 Human
- Unigene: 10595 Rat
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Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/50 dilution. The image on the right is treated blocking peptide.
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All lanes : Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111) at 1/500 dilution
Lane 1 : MCF7 cell extracts, untreated
Lane 2 : MCF7 cell extracts, treated with EGF
Predicted band size: 66 kDa
Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking. -
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131111 has not yet been referenced specifically in any publications.