Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S106) antibody
    See all Estrogen Receptor alpha primary antibodies
  • Description

    Rabbit polyclonal to Estrogen Receptor alpha (phospho S106)
  • Host species

    Rabbit
  • Specificity

    ab131111 detects Estrogen Receptor alpha only when phosphorylated at serine 106.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of serine 106 (S-P-S(p)-P-L) derived from Human Estrogen Receptor alpha (NP_000116.2).

  • Positive control

    • MCF cells; MCF7 cell extracts treated with EGF; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/50 dilution. The image on the right is treated blocking peptide.
  • All lanes : Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111) at 1/500 dilution

    Lane 1 : MCF7 cell extracts, untreated
    Lane 2 : MCF7 cell extracts, treated with EGF

    Predicted band size: 66 kDa



    Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking.
  • Immunoflourescent analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/100 dilution.

References

ab131111 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium citrate, 0.05% Tween 20, pH 6.0
Permeabilization
No
Specification
Uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 24 2018

Application
Western blot
Sample
Rat Cell lysate - whole cell (Mammary epithelial cells)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
50 µg
Specification
Mammary epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 28 2018

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