Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S106)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Estrogen Receptor alpha (phospho S106) antibody
See all Estrogen Receptor alpha primary antibodies
DescriptionRabbit polyclonal to Estrogen Receptor alpha (phospho S106)
Specificityab131111 detects Estrogen Receptor alpha only when phosphorylated at serine 106.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of serine 106 (S-P-S(p)-P-L) derived from Human Estrogen Receptor alpha (NP_000116.2).
- MCF cells; MCF7 cell extracts treated with EGF; Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131111 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab131111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/50 dilution. The image on the right is treated blocking peptide.
All lanes : Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111) at 1/500 dilution
Lane 1 : MCF7 cell extracts, untreated
Lane 2 : MCF7 cell extracts, treated with EGF
Predicted band size: 66 kDa
Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking.
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/100 dilution.
ab131111 has not yet been referenced specifically in any publications.