Product nameAnti-Estrogen Receptor alpha (phospho S106) antibody
See all Estrogen Receptor alpha primary antibodies
DescriptionRabbit polyclonal to Estrogen Receptor alpha (phospho S106)
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Estrogen Receptor alpha (phospho S106). A phospho specific peptide corresponding to residues surrounding S106 of Human Estrogen Receptor alpha.
Database link: P03372
- EGF-treated MCF7 cell lysate; Human breast carcinoma tissue and MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/100 - 1/200.|
|IHC-P||1/50 - 1/100.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
All lanes : Anti-Estrogen Receptor alpha (phospho S106) antibody (ab194784) at 1/500 dilution
Lane 1 : MCF7 cells
Lane 2 : lysate of MCF7 cells treated with EGF
Predicted band size: 66 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast carcinoma tissue, labeling Estrogen Receptor alpha (phospho S106) using ab194784 at a 1/50 dilution.
ab194784 has not yet been referenced specifically in any publications.