Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S106)
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Estrogen Receptor alpha (phospho S106) antibody
See all Estrogen Receptor alpha primary antibodies
DescriptionRabbit polyclonal to Estrogen Receptor alpha (phospho S106)
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Estrogen Receptor alpha (phospho S106). A phospho specific peptide corresponding to residues surrounding S106 of Human Estrogen Receptor alpha.
Database link: P03372
- WB: EGF-treated MCF-7 cell lysate; IHC-P: Human breast carcinoma tissue; ICC/ IF: MCF-7 cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/100 - 1/200.|
|IHC-P||1/50 - 1/100.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
All lanes : Anti-Estrogen Receptor alpha (phospho S106) antibody (ab194784)
Lane 1 : MCF-7 cell lysate
Lane 2 : lysate of MCF-7 cells treated with EGF
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 66 kDa
Blocking buffer: 3% BSA.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, labeling Estrogen Receptor alpha (phospho S106) using ab194784.
Immunofluorescence staining of MCF-7 cells stained for Estrogen Receptor alpha (phospho S106) with ab194784.
ab194784 has not yet been referenced specifically in any publications.