Recombinant
RabMAb

Recombinant Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

Submit a review Submit a question

Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free
    See all Estrogen Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blotmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Estrogen Receptor alpha. The exact sequence is proprietary.

  • General notes

    Ab177817 is the carrier-free version of ab32396. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab177817 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab177817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    • Post-translational
      modifications

      Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).

    • Cellular localization

      Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Information by UniProt

    • Database links

      • Alternative names

        • 7*/654 isoform antibody
        • 7*/819 2 isoform antibody
        • 7*/822 isoform antibody
        • 8*/901 isoform antibody
        • 8*/941 isoform antibody
        • DKFZp686N23123 antibody
        • ER alpha antibody
        • ER antibody
        • ER-alpha antibody
        • Era antibody
        • ESR antibody
        • ESR1 antibody
        • ESR1_HUMAN antibody
        • ESRA antibody
        • Estradiol receptor antibody
        • Estrogen nuclear receptor alpha antibody
        • Estrogen receptor 1 antibody
        • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
        • Estrogen receptor alpha antibody
        • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
        • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
        • Estrogen receptor alpha delta 4 +49 isoform antibody
        • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
        • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
        • Estrogen receptor alpha E1 E2 1 2 antibody
        • Estrogen receptor alpha E1 N2 E2 1 2 antibody
        • Estrogen receptor antibody
        • ESTRR antibody
        • NR3A1 antibody
        • Nuclear receptor subfamily 3 group A member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) treated with EGF (100ng/ml, 5min) and treated with Lambda Protein Phosphatase 31? for 2h cells labeling Estrogen receptor alpha (phospho S118) with purified ab32396 at 1:200 dilution (8.9μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 5 μg/ml (1/200 dilution). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor®488 Goat anti-Rabbit  was used as the secondary antibody at 2 μg/ml (1/1000 dilution). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 2.5 μg/ml (1/200 dilution). DAPI nuclear counterstain.
        Confocal image showing the signal increased after EGF (100ng/ml, 5 min) treatment and decreased after Lambda Protein Phosphatase treatment (31°C for 2 hours).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Dot blot analysis of Lane 1: Estrogen Receptor alpha (pS118) phospho peptide and Lane 2: Estrogen Receptor alpha non-phospho peptide labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunofluorescent staining of (A) untreated and (B) Phosphatase-treated MCF-7 cells using unpurified ab32396.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-embedded human breast adenocarcinoma using unpurified ab32396 at 1:50 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      References

      ab177817 has not yet been referenced specifically in any publications.

      Publishing research using ab177817? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab177817.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com