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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Estrogen
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RecombinantRabMAb

Recombinant Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
  • Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
  • Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118) - BSA and Azide free
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Human

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Protein
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Recombinant Human Estrogen Receptor alpha protein (ab82606)

View more associated products

Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free
    See all Estrogen Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118) - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab177817 is the carrier-free version of ab32396. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab177817 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E91
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen

Associated products

  • Alternative Versions

    • Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)
  • Assay kits

    • Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (ab207203)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Estrogen Receptor alpha protein (ab82606)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Post-translational
      modifications

      Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).
    • Cellular localization

      Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Target information above from: UniProt accession P03372 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2099 Human
      • Omim: 133430 Human
      • SwissProt: P03372 Human
      • Unigene: 208124 Human
      • Alternative names

        • 7*/654 isoform antibody
        • 7*/819 2 isoform antibody
        • 7*/822 isoform antibody
        • 8*/901 isoform antibody
        • 8*/941 isoform antibody
        • DKFZp686N23123 antibody
        • ER alpha antibody
        • ER antibody
        • ER-alpha antibody
        • Era antibody
        • ESR antibody
        • ESR1 antibody
        • ESR1_HUMAN antibody
        • ESRA antibody
        • Estradiol receptor antibody
        • Estrogen nuclear receptor alpha antibody
        • Estrogen receptor 1 antibody
        • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
        • Estrogen receptor alpha antibody
        • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
        • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
        • Estrogen receptor alpha delta 4 +49 isoform antibody
        • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
        • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
        • Estrogen receptor alpha E1 E2 1 2 antibody
        • Estrogen receptor alpha E1 N2 E2 1 2 antibody
        • Estrogen receptor antibody
        • ESTRR antibody
        • NR3A1 antibody
        • Nuclear receptor subfamily 3 group A member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) treated with EGF (100ng/ml, 5min) and treated with Lambda Protein Phosphatase 31? for 2h cells labeling Estrogen receptor alpha (phospho S118) with purified ab32396 at 1:200 dilution (8.9μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 5 μg/ml (1/200 dilution). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor®488 Goat anti-Rabbit  was used as the secondary antibody at 2 μg/ml (1/1000 dilution). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 2.5 μg/ml (1/200 dilution). DAPI nuclear counterstain.
        Confocal image showing the signal increased after EGF (100ng/ml, 5 min) treatment and decreased after Lambda Protein Phosphatase treatment (31°C for 2 hours).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Dot blot analysis of Lane 1: Estrogen Receptor alpha (pS118) phospho peptide and Lane 2: Estrogen Receptor alpha non-phospho peptide labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunofluorescent staining of (A) untreated and (B) Phosphatase-treated MCF-7 cells using unpurified ab32396.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

        Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-embedded human breast adenocarcinoma using unpurified ab32396 at 1:50 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32396).

      • Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)
        Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] - BSA and Azide free (ab177817)

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab177817? Please let us know so that we can cite the reference in this datasheet.

      ab177817 has not yet been referenced specifically in any publications.

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