Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Estrogen Receptor alpha (phospho S167) antibody
See all Estrogen Receptor alpha primary antibodies
DescriptionRabbit polyclonal to Estrogen Receptor alpha (phospho S167)
Specificityab131105 detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at serine 167.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- MCF7 cell extract; MCF cells; Human breast carcinoma tissue
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131105 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 66 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Estrogen Receptor alpha (phospho S167) with ab131105 at 1/50 dilution. Right panel was preincubated with blocking peptide.
All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105) at 1/500 dilution
Lane 1 : MCF7 cell extract with blocking peptide
Lane 2 : MCF7 cell extract
Predicted band size: 66 kDa
Immunofluorescence analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S167) with ab131105 at 1/100 dilution.
ab131105 has not yet been referenced specifically in any publications.